Sod activity kit

Cardiomyocyte SOD activity was determined by the SOD activity kit (Beyotime) [23 (link)]. Briefly, cardiomyocytes that received different interventions were lysed, and the lysates were 2-(4-iodophenyl)-3-(4-nitrophenol)-5- phenyltetrazolium chloride followed by measuring the SOD activity with determining absorbance at 505 nm.

2FF was provided by SynChem. Inc. (IL, United States). NAC was purchased from Sangon Biotech (Shanghai) Co., LTD. (Shanghai, China). Acetaminophen was provided by Abcam (CA, United States). The aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutathione (GSH) and malondialdehyde (MDA) test kits were provided by Jiancheng Bioengineering Institute (Nanjing, China). The lactate dehydrogenase (LDH) kit, MTT cell proliferation and cytotoxicity kit and SOD activity kit were provided by Beyotime Biotechnology (Nantong, China). Reactive Oxygen Species (ROS) Fluorescent Probe-Dihydroethidium (DHE) was provided by Vigorousbio. CO, Ltd. (Beijing, China). The mouse TNF-α and IL-6 ELISA kits were obtained from ABclonal Technology Co., Ltd. (Wuhan, China). Aleuria aurantia lectin (AAL), wheat germ agglutinin (WGA) and concanavalin A (ConA) were provided by Vectorlabs, Inc. (CA, United States). Antibodies against Nrf2, NQO1, keap1, NF-κB p65 and iNOS were from Abcam (Cambridge, United Kingdom). Antibodies against glyceraldehyde-3-phosphate dehydrogenase (GAPDH), COX-2, HO-1 and Histone were from Proteintech (Wuhan, China).