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Mouseanti psd95 clone 6g6 1c9

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Mouseanti-PSD95 (clone 6G6-1C9) is a primary antibody that specifically binds to the PSD-95 protein in mouse samples. PSD-95 is a scaffolding protein found in the postsynaptic density of neuronal cells.

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Lab products found in correlation

Bis tris gel, by Bio-Rad (2 mentions) Image reader las 4000, by Fujifilm (2 mentions) Ripa buffer, by Thermo Fisher Scientific (2 mentions) Rabbit anti β actin, by Merck Group (2 mentions) Protease phosphatase inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Wasperoxidase labelled anti rabbit igg, by Vector Laboratories (2 mentions)

2 protocols using mouseanti psd95 clone 6g6 1c9

1

Quantifying Synaptic Proteins in Cortical Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein was extracted from cortical tissue by homogenization in RIPA
buffer (Thermo Fisher Scientific) containing phosphatase/protease inhibitor
cocktail (Thermo Fisher Scientific) and centrifuged at 15,000 ×
g for 20 minutes at 4°C before collecting the
supernatant. A 50 μg aliquot of total protein was
separated by electrophoresis in precast 4% to 12% Bis-Tris Gels (Bio-Rad) and
transferred to nitrocellulose membranes. Membranes were blocked in 5% milk
before probing with the following antibodies: rabbit anti-SHIP1 (1:1000, Cell
Signaling, D1163), rabbit anti-β-actin (1:2500, Sigma, A-2066), mouse
anti-PSD95 (clone 6G6-1C9, 1:2500, EMD Millipore, MAB1596), and rabbit
anti-synaptophysin (1:1000, Abcam, ab16659). The secondary antibody used was
peroxidase-labelled anti-rabbit IgG (1:5000, Vector Laboratories). SuperSignal
West Femto ECL (Pierce, #34096) was used to reveal the immunoreactive proteins,
and images were acquired using a Fujifilm ImageReader LAS-4000. Luminescent
immunoreactive protein bands were quantified using Fiji software (ImageJ).
, & Moulton D. (2001). Will new NS law lead to bidding wars for MDs?. CMAJ: Canadian Medical Association Journal, 165(3), 327.
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2

Quantifying Synaptic Proteins in Cortical Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein was extracted from cortical tissue by homogenization in RIPA
buffer (Thermo Fisher Scientific) containing phosphatase/protease inhibitor
cocktail (Thermo Fisher Scientific) and centrifuged at 15,000 ×
g for 20 minutes at 4°C before collecting the
supernatant. A 50 μg aliquot of total protein was
separated by electrophoresis in precast 4% to 12% Bis-Tris Gels (Bio-Rad) and
transferred to nitrocellulose membranes. Membranes were blocked in 5% milk
before probing with the following antibodies: rabbit anti-SHIP1 (1:1000, Cell
Signaling, D1163), rabbit anti-β-actin (1:2500, Sigma, A-2066), mouse
anti-PSD95 (clone 6G6-1C9, 1:2500, EMD Millipore, MAB1596), and rabbit
anti-synaptophysin (1:1000, Abcam, ab16659). The secondary antibody used was
peroxidase-labelled anti-rabbit IgG (1:5000, Vector Laboratories). SuperSignal
West Femto ECL (Pierce, #34096) was used to reveal the immunoreactive proteins,
and images were acquired using a Fujifilm ImageReader LAS-4000. Luminescent
immunoreactive protein bands were quantified using Fiji software (ImageJ).
Castranio E.L., Hasel P., Haure-Mirande J.V., Ramirez Jimenez A.V., Hamilton B.W., Kim R.D., Glabe C.G., Wang M., Zhang B., Gandy S., Liddelow S.A, & Ehrlich M.E. (2022). Microglial INPP5D limits plaque formation and glial reactivity in the PSAPP mouse model of Alzheimer’s disease. Alzheimer's & dementia : the journal of the Alzheimer's Association, 19(6), 2239-2252.
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