Proteins from cells were extracted and separated by SDS‐PAGE. After transferred onto PVDF membranes (Millipore), the proteins were blocked with 5% skim milk and then incubated with Bax antibody (dilution 1:800; Abcam), cleaved caspase 3 antibody (dilution 1:800; Abcam), Bcl‐2 antibody (dilution 1:800; Abcam), HMGA2 antibody (dilution 1:800; Abcam) or GAPDH antibody (dilution 1:2000; Abcam) at 4℃ overnight. After washed with TBST buffer, the membranes were incubated with HRP‐conjugated secondary antibodies (dilution 1:5000; Beyotime) and the bands were visualized using BeyoECL Moon (Beyotime).
Bax antibody
Manufactured by Abcam
Sourced in United States
Bax antibody is a product that recognizes the Bax protein, a member of the Bcl-2 family of proteins. Bax plays a crucial role in the regulation of apoptosis, or programmed cell death. The antibody can be used for the detection and analysis of Bax expression in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Bcl 2 antibody, by Abcam (8 mentions)
Gapdh antibody, by Abcam (4 mentions)
Caspase 3 antibody, by Abcam (2 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Hrp conjugated secondary antibody, by Beyotime (1 mentions)
Cleaved caspase 3 antibody, by Abcam (1 mentions)
Beyoecl moon, by Beyotime (1 mentions)
Hmga2 antibody, by Abcam (1 mentions)
Goat anti rabbit igg secondary antibody, by ZSGB-BIO (1 mentions)
Caspase 3 antibody, by Santa Cruz Biotechnology (1 mentions)
Gel imaging system, by Analytik Jena (1 mentions)
β actin antibody, by ZSGB-BIO (1 mentions)
β catenin antibody, by Abcam (1 mentions)
Twist antibody, by Abcam (1 mentions)
E cadherin antibody, by Abcam (1 mentions)
N cadherin antibody, by Abcam (1 mentions)
Snail antibody, by Abcam (1 mentions)
Gsk3β antibody, by Abcam (1 mentions)
Reactive oxygen species assay kit, by Beyotime (1 mentions)
Atp assay kit, by Beyotime (1 mentions)
12 protocols using bax antibody
1
Protein Expression Analysis by Western Blot
2
Myocardial Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The left ventricle wall was collected and rapidly frozen in liquid nitrogen and stored at −80°C. The heart was removed from a −80°C freezer, and the left ventricular myocardial tissue was sheared on ice. The myocardial tissue was homogenized to directly collect the supernatant liquor. The protein sample was mixed with buffer and heated to denaturation, and then SDS-PAGE was added. The sample was transferred to a polyvinylidene fluoride (PVDF) membrane, sealed with a sealing solution for 1 h, and then incubated overnight at 4°C with the corresponding primary antibodies, including p-PI3K antibody (Bioss, China, 1 : 600), p-Akt antibody (Bioworld, China, 1 : 600), Bad antibody (Bioss, China, 1 : 600), Bcl-2 antibody (Abcam, UK, 1 : 500), Bax antibody (Abcam, UK, 1 : 1000), Caspase-3 antibody (Santacruz, USA, 1 : 400), and β-actin antibody (ZSGB-BIO, China, 1 : 1000). The reaction membrane was incubated with secondary goat anti-rabbit IgG antibody (ZSGB-BIO, China, 1 : 3000) at room temperature for 1 h. The ECL luminescence kit (Santacruz, USA) was used for colorimetric detection. Images were scanned and measured in terms of the grayscale values by the gel imaging system (UVP, USA).
3
Evaluating EMT and Apoptosis Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cells of each group were collected and lysed, while the total protein was extracted. The protein concentration was examined by BCA, and we adjusted the protein concentration of each group. We loaded samples 30 μg/well, and electrophoresis was performed with SDS-PAGE gel. The protein was transferred to the PVDF membrane, sealed with skim milk at 25°C, incubated with primary antibody at 4°C overnight, incubated with HRP-linked secondary antibody, developed and photographed with an ECL kit. Primary antibodies included Snail antibody (ab216347), Twist antibody (ab50887), E-cadherin antibody (ab231303), N-cadherin antibody (ab76011), GSK3β antibody (ab32391), β-catenin antibody (ab32572), Bax antibody (ab32503), Bcl-2 antibody (ab182858), and GAPDH antibody (ab8245), all derived from Abcam.
4
Mitochondrial Function and Apoptosis Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AS-IV (batch number: 140913) was obtained from Weikeqi Biotechnology (Sichuan, China). Betloc tablets (batch number: 2010038) were purchased from AstraZeneca (Wuxi, China). Cell Counting Kit-8 (CCK-8, CAS Number: ZP328-3) was purchased from Zomanbio (Beijing, China). ATP Assay kit (CAS Number: S0026), mitochondrial membrane potential assay kit with JC-1 (CAS Number: C2006), and reactive oxygen species assay kit (CAS Number: S0033S) were purchased from Beyotime Biotechnology (Shanghai, China). RiboFECT transfection kit (CAS Number: C10511-1) was obtained from Ribo Biotechnology (Guangzhou, China). Mitochondrial isolation kit (Lot#019M4159V) was purchased from Sigma (St. Louis, USA). Troponin I antibody (CAS Number: ab209809), CD31 antibody (CAS Number: ab222783), α-smooth muscle actin (α-SMA) antibody (CAS Number: ab124964), BAX antibody (CAS Number: ab32503), Bcl2 antibody (CAS Number: ab196495), cytochrome C antibody (CAS Number: ab133504), Sirt3 antibody (CAS Number: ab246522), Opa1 antibody (CAS Number: ab42364), VDAC1 antibody (CAS Number: ab15895), and Drp1 antibody (CAS Number: ab184247) were obtained from Abcam (Cambridge, MA, USA).
5
Protein Expression Analysis in Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LNCaP and DU145 cells were lysed with ice-cold RIPA lysis buffer (Millipore) and total protein was collected. Subsequently, protein concentration was detected by a BCA Protein Assay Kit (HyClone-Pierce). Equal amount proteins (20 µg) were separated by 10% SDS-PAGE (Invitrogen) and transferred to PVDF membrane (BIO-RAD). The membrane was blocked in TBST solution containing 5% non-fat milk for 1 h and incubated with primary antibodies (CACYBP antibody, 1:3000, Cat. #ab171972, Abcam; p53 antibody, 1:2000, Cat. #10442-1-AP, Proteintech; p-p53 antibody, 1:2000, Cat. #28961-1-AP, Proteintech; Bax antibody, 1:2000, Cat. #ab182733, Abcam; Bcl-2 antibody, 1:2000, Cat. #ab182858, Abcam; GAPDH antibody, 1:3000, Cat. #60004-1-lg, Proteintech) at room temperature for 2 h. Then, the membrane was continuingly incubated with the secondary antibody Goat Anti-Rabbit (1:3000, Cat. #A0208, Beyotime) at room temperature for 1 h. Protein bands were visualized by ECL plus TM Western blotting system kit (Millipore).
6
Cardiac Tissue Protein Extraction and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The total protein of mouse cardiac tissues and cells was extracted with the Pierce IP Lysis Buffer (Thermo Fisher Scientific Inc.). The concentration of proteins was measured using the Pierce BCA Protein Assay Kit (Thermo Scientific). Equal amounts of extracted protein (60 μg) were separated by 10% SDS‐PAGE and were transferred to PVDF membranes. The membranes were blocked with 5% non‐fat milk for 2 hours and incubated with primary antibodies: Bcl‐2 antibody, Bax antibody and NF‐κB antibody (Abcam, Cambridge, MA, USA) at 4°C overnight and incubated with HRP‐conjugated secondary antibodies at room temperature for 1 hour. GAPDH antibody was used as the internal control. The bands were analysed using the Enhanced Chemiluminescence Kit (GE Healthcare, Chicago, IL, USA).
7
Evaluation of Colitis Serum Toxicity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the high dose treatment of JW, we collected the serum from colitis mice in two hours for the following experiments. To examine the toxicity, NCM460 cells, an epithelial cell line derived from the normal colon of a 68-year-old Hispanic male, were treated with the serum and subjected to an MTT assay (3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) and cellular immunofluorescence assay with BAX antibody (Abcam, USA).
8
Immunohistochemical Analysis of KIF15 and Tumor Markers in Glioblastoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The expression of KIF15 was detected by immunohistochemistry in glioblastoma tissues. Tumor and para-carcinoma tissue sections from glioblastoma patients were deparaffinized. Following citrate antigen repair and blocking by 3% H2O2 for 10 min at room temperature, the samples were incubated with the KIF15 antibody (1:50; Fine Test) at 4°C overnight in an incubator. The sections were subsequently incubated with goat anti-rabbit IgG H&L horseradish peroxidase (HRP)-conjugated secondary antibody (1:400; cat. no. ab6721; Abcam) at 37°C for 1 h. Images were captured by a light microscope (Olympus Corp.). Ki-67 antibody (1:100; cat. no. ab16667; Abcam), Bax antibody (1:250; cat. no. ab32503; Abcam), p21 antibody (1:50; cat. no. BM3990; BOSTER) and Survivin antibody (1:100; cat. no. ab469; Abcam) were used in the immunohistochemistry analysis of tumor sections removed from mice models.
9
DU145 Prostate Cancer Cell Line
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The prostate cancer DU145 cell line was provided by Utilico (Shanghai) Life Sciences Ltd. (item No. YLK-XB127). The suppliers of the main were reagents were: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent (Nanjing KGI, China); taurine (Merck Sigma, USA); MST1 antibody, YAP antibody, Bax antibody, Bcl-2 antibody, horseradish peroxidase (HRP)-labeled secondary antibody (Abcam, USA).
10
Apoptosis and EMT Regulation Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies against Bcl-2 and β-actin were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Antibodies against iNOS, Bcl-xL, poly-ADP ribose polymerase (PARP), cleaved-PARP, caspase-3, cleaved caspase-3, caspase-9, cleaved caspase-9, E-cadherin, N-cadherin, vimentin, snail, and twist were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Nrf2 and Bax antibodies were purchased from Abcam, Inc. (Cambridge, MA, USA). Annexin V assay for apoptosis analysis was performed using the FITC Annexin V apoptosis detection kit I (BD Biosciences, San Diego, CA, USA). Transwell chambers with 8 µm pore size polycarbonate membrane inserts and Matrigel were obtained from Corning (Costar Corning Inc., NY, USA) and BD Biosciences (San Diego, CA, USA), respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!