Ph2ax s139 clone jbw301

Manufactured by Merck Group

Sourced in United States

PH2AX (S139) (clone JBW301) is a lab equipment product from Merck Group. It is a monoclonal antibody that recognizes phosphorylated serine 139 on the histone variant H2AX, which is a marker of DNA double-strand breaks.

Automatically generated - may contain errors

Lab products found in correlation

Prpa32 s4 s8, by Fortis Life Sciences (3 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Geminin, by Cell Signaling Technology (2 mentions) Dna pkcs, by Cell Signaling Technology (2 mentions) Pdna pkcs s2056, by Cell Signaling Technology (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Rpa32, by Abcam (1 mentions) Proliferating cell nuclear antigen (pcna), by Cell Signaling Technology (1 mentions) P rbs807 s811, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

JBW301 (109 citations) Clone JBW301 (14 citations) PH2AX (13 citations)

3 protocols using ph2ax s139 clone jbw301

Immunoblotting Analysis of Cell Cycle Regulators

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Antibodies against Chk1, pChk1 (S317), pChk2 (T68), pH2AX (S139), pCdc2 (Y15), pHH3 (S10), PCNA, CDT1, MCM2, Geminin and GAPDH were purchased from Cell Signaling Technologies; pChk1 (S296), RPA32 and Cdc6 from Abcam; pRPA32 (S4/S8) from Bethyl Laboratories and pH2AX (S139) (clone JBW301) from Merck Millipore. Antibodies were used at the manufacturer’s recommended dilutions.

, & Massey A.J. (2017). Modification of tumour cell metabolism modulates sensitivity to Chk1 inhibitor-induced DNA damage. Scientific Reports, 7, 40778.

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Antibody Detection of DNA Damage Response

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Antibodies against Chk1, pChk1 (S317), pChk2 (T68), pChk2 (S516), pH2AX (S139), pCdc2 (Y15), pHH3 (S10), GAPDH, DNA-PKcs, pDNA-PKcs (S2056), and cleaved Caspase-3 were purchased from Cell Signaling Technologies (Danvers, USA); pChk1 (S296), RPA32, RPA70 and BrdU (clone MoBu-1) from Abcam (Cambridge, UK); pRPA32 (S4/S8) from Bethyl Laboratories (Montgomery, USA) and pH2AX (S139) (clone JBW301) from Merck Millipore (Watford, UK). Antibodies were used at the manufacturer's recommended dilutions.

Wayne J., Brooks T, & Massey A.J. (2016). Inhibition of Chk1 with the small molecule inhibitor V158411 induces DNA damage and cell death in an unperturbed S-phase. Oncotarget, 7(51), 85033-85048.

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Antibody Assay for Cell Cycle Markers

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Antibodies against pChk1 (S296), Cdc6, E2F1, RPA32 and RRM2, and were purchased from Abcam; pCDK1/2 (Y15), CDT1, pChk1 (S317), Chk1, pChk2 (T68), Chk2, Cyclin A2, Cyclin E1, p DNA-PKcs (S2056), DNA-PKcs, GAPDH, Geminin, pH2AX (S139), pHH3 (S10), Ki67, MCM2, PCNA, pRb (S807/S811) and Rb from Cell Signaling Technologies; pRPA32 (S4/S8) from Bethyl Laboratories and pH2AX (S139) (clone JBW301) from Merck Millipore. Antibodies were used at the manufacturer’s recommended dilutions.

, & Massey A.J. (2016). Tumour growth environment modulates Chk1 signalling pathways and Chk1 inhibitor sensitivity. Scientific Reports, 6, 35874.

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