OVCAR3 and SKOV3 cells were obtained from Cell Bank of Chinese Academy of Sciences (Shanghai, China) and maintained in RPMI-1640 medium (HyClone, USA) supplemented with 10% FBS at 37 °C with 5% CO2. Cells were transfected with pCMV-MIR-miR-454 (5 μg; Ribobio, Guangzhou, China) using Lipofectamine 2000 (Invitrogen, CA, USA) according to the instructions, pCMV-MIR vector (5 μg; Ribobio) was used as negative control (NC). The E2F6 cDNA sequences were cloned into pcDNA3.1 vector and the pcDNA3.1-E2F6 (5 μg; Ribobio) was transfected into cells using Lipofectamine 2000.
Pcdna3.1 e2f6
Manufactured by RiboBio
PcDNA3.1-E2F6 is a plasmid vector that expresses the E2F6 transcription factor. It is commonly used in research applications to study the role of E2F6 in cellular processes.
Automatically generated - may contain errors
2 protocols using pcdna3.1 e2f6
1
Overexpression of miR-454 and E2F6 in Ovarian Cancer
2
Overexpression of miR-454 and E2F6 in Ovarian Cancer
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Cell lines and cell culture OVCAR3 and SKOV3 cells were obtained from Cell Bank of Chinese Academy of Sciences (Shanghai, China) and maintained in RPMI-1640 medium (HyClone, USA) supplemented with 10% FBS at 37°C with 5% CO 2 . Cells were transfected with pCMV-MIR-miR-454 (5μg; Ribobio, Guangzhou, China) using Lipofectamine 2000 (Invitrogen, CA, USA) according to the instructions, pCMV-MIR vector (5μg; Ribobio) was used as negative control (NC). The E2F6 cDNA sequences were cloned into pcDNA3.1 vector and the pcDNA3.1-E2F6 (5μg; Ribobio) was transfected into cells using Lipofectamine 2000.
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