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Typer analyzer software

Manufactured by Agena

The Typer Analyzer software is a laboratory tool designed to analyze and interpret data from various types of analytical instruments. It provides a platform for processing and visualizing experimental results, enabling users to efficiently manage and understand their data.

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6 protocols using typer analyzer software

1

Genetic Variant Profiling via PCR-SBE

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Specific DNA fragments were amplified using genomic DNA in three PCR reactions and subsequently alleles were interrogated using SBE. Genotypes were detected using a MassARRAY® Analyzer 4 system and haplotypes assigned using an ADME CYP2C9/VKORC1 Reporter plugin for the Typer Analyzer software (Agena Bioscience, San Diego, CA).
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2

Genotyping via PCR and SBE

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Specific DNA fragments were amplified using genomic DNA in two PCR reactions and subsequently alleles were interrogated using SBE. Genotypes were detected using a MassARRAY® Analyzer 4 system and haplotypes assigned using an ADME PGx Pro Reporter plugin for the Typer Analyzer software (Agena Bioscience, San Diego, CA)
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3

PCR-based Genotyping and ADME Analysis

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A specific DNA fragment was generated using PCR and genomic DNA and alleles were subsequently interrogated using a homogeneous mass extension reaction. Genotypes were detected using a MassARRAY® Analyzer 4 system and assigned manually using the Typer Analyzer software (Agena Bioscience, San Diego, CA). The TA repeat genotypes were also incorporated into the ADME PGx Reporter output using a modified ADME PGx Pro database.
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4

Genotyping SNPs Associated with Obesity

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We sought to genotype all SNPs that reached genome-wide levels of significance in the largest (at the time of genotyping) GWAS of BMI (5 (link)) and WHR (6 (link)). The iPLEX GOLD procedure was followed for the SNP analysis. Briefly, Sequenom’s Assay Design software was used to create five multiplexed reactions for all SNPs of interest. After extending the primers the products were conditioned and spotted onto SpectroChip Arrays. Extended primers were detected by MassArray Analyzer 4 using matrix-assisted laser desorption ionization–time-of-flight (MALDITOF) method. Data was acquired using the TyperAnalyzer Software from Agena Bioscience (formerly Sequenom). During the design phase of the assay, it was determined that some SNPs would not genotype well on the system and the SNAP browser was used to pick suitable proxies in the Caucasian population. All SNPs having genotyping rates below 95% were discarded, as were SNPs with HWE p-values less than .01. 34 of 46 SNPs passed these quality control filters. The rs numbers of these SNPs are included in a footnote to Table 1.
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5

Comprehensive Pharmacogenomic Profiling

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All samples were genotyped for 67 positions (Table 1) using iPLEX Pro chemistry on the MassARRAY® Analyzer 4 System (Agena Biosciences, San Diego, CA, USA). Twenty-nine SNP and indel variants as well as copy number status of the CYP2D6 gene were analyzed using the Agena iPLEX® ADME CYP2D6 Panel v1.0, which is a 3-well assay combining genotyping for SNP and indel variants as well as five assays to determine genomic copy number. This genotyping platform and assay have been previously shown to accurately genotype pharmacogenetic loci in Coriell reference samples.32 (link)CYP2D6 diplotypes and CNV calling for MassArray data were determined using the Agena PGx Report 2.0 Reporter plugin for the Typer Analyzer software (Agena Bioscience). CYP2D6 copy number was estimated from the five copy number assays that are integrated in the CYP2D6 genotyping panel, which is calculated from informative polymorphisms between CYP2D6 and CYP2D7. Variants in the remaining 18 genes in this study were also typed on the MassARRAY® Analyzer 4 System with custom-designed primers using a combination of Agena’s Assay Design Suite (ADS) and Primer3 (ref 33 (link)).
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6

Comprehensive Genotyping of UGT1A1 Alleles

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Specific DNA fragments were amplified from genomic DNA in eight PCR reactions and alleles were subsequently interrogated using single base extension (SBE) reactions. Genotypes were detected using a MassARRAY® Analyzer 4 system and haplotypes assigned using an ADME PGx Pro Reporter plugin for the Typer Analyzer software (Agena Bioscience, San Diego, CA). The UGT1A1 TA repeat assay genotypes were also incorporated into the ADME PGx Reporter output using a modified ADME PGx Pro database (http://www.biotechniques.com/protocols/2012_Protocol_Guide/Development-and-Research-Validation-of-the-iPLEX----ADME-PGx-Panel-on-the-MassARRAY-System/biotechniques-330915.html, last accessed April 17, 2015).
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