Cd45 clone k252.1e4

Manufactured by Bio-Rad

Sourced in United Kingdom, United States

CD45 (clone K252.1E4) is a laboratory reagent used for the identification and characterization of cells. It is a monoclonal antibody that specifically binds to the CD45 antigen, which is expressed on the surface of various hematopoietic cells, including lymphocytes, monocytes, and granulocytes. The core function of this product is to facilitate the detection and analysis of these cell types in research and clinical applications.

Automatically generated - may contain errors

Lab products found in correlation

Cd16 clone g7, by Bio-Rad (2 mentions) Cd49d clone l25, by BD (1 mentions)

2 protocols using cd45 clone k252.1e4

Analyzing PMN Receptor Expression

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For the analysis of PMN receptor expression by flow measurements the following anti-pig antibodies were commercially purchased: CD45 (clone K252.1E4, Abd Serotec, Kidlington, UK), SWC8 (clone MIL-1, AbD Serotec, Kidlington, UK), CD11b(RIII) (clone 2F4/11, AbD Serotec, Kidlington, UK), CD16 (clone G7, Abd Serotec, Kidlington, UK), CD62L (clone produced by Laboratory for Translational Research, Utrecht) and CD32 (Clone AT10, AbD Serptec, Kidlington, UK).

Teuben M.P., Pfeifer R., Horst K., Simon T.P., Heeres M., Kalbas Y., Blokhuis T., Hildebrand F., Koenderman L., Pape H.C, & Leenen L. (2021). Standardized porcine unilateral femoral nailing is associated with changes in PMN activation status, rather than aberrant systemic PMN prevalence. European Journal of Trauma and Emergency Surgery, 48(3), 1601-1611.

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Multiparametric Neutrophil Receptor Profiling

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For the flow cytometry analysis of neutrophil receptor expression, the following commercially available anti-pig monoclonal antibodies were obtained and when indicated conjugated by validated and commercially available antibody conjugation kits: SWC 1 (clone K263.3d7, Novus Biol, Centennial, Colorado, USA), SWC 3 (clone 74-22-15, Accurate Chemical, Westbury, New York, USA), SWC 8 (MIL-1, Abd Serotec, Kidlington, UK), CD11b (clone 2F4/11, Abd Serotec, Kidlington, UK), CD16 (clone G7, Abd Serotec, Kidlington, UK), CD29 (clone NaM160-1A3, BD, Mountain View, CA, USA), CD45 (clone K252.1E4, Abd Serotec, Kidlington, UK), CD45Ra (clone MIL-13, GenWay Biotech, San Diego, CA, USA), CD49D (clone L25, BD, Mountain View, CA, USA), CD184 (clone H-118, Santa Cruz Biotechnol, Santa Cruz, CA, USA). Lightning Link conjugation kits (Novus Biol, Centennial, Colorado, USA). A viability staining Vivid (Invitrogen, Waltham, USA) was added to exclude dead cells.

Teuben M.P., Heeres M., Blokhuis T., Spijkerman R., Knot E., Vrisekoop N., Pfeifer R., Pape H.C., Koenderman L, & Leenen L.P. (2022). Shift of Neutrophils From Blood to Bone Marrow Upon Extensive Experimental Trauma Surgery. Frontiers in Immunology, 13, 883863.

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