Agilent whole mouse gene expression microarray

Manufactured by Agilent Technologies

Sourced in United States

The Agilent Whole Mouse Gene Expression Microarray is a laboratory equipment designed for the analysis of gene expression in mice. It provides a comprehensive platform for researchers to study the expression levels of thousands of mouse genes simultaneously.

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Lab products found in correlation

Low rna input linear amplification kit, by Agilent Technologies (2 mentions) Feature extraction 10, by Agilent Technologies (2 mentions) Agilent scanner, by Agilent Technologies (2 mentions)

Spelling variants of this product

Agilent microarrays (24 citations) Gene expression microarrays (4 citations) Whole‐mouse gene expression microarray (2 citations)

2 protocols using agilent whole mouse gene expression microarray

Whole Mouse Gene Expression Microarray Analysis

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RNA was hybridized to an Agilent Whole Mouse Gene Expression Microarray (G4852A, 8 × 60K; Agilent Technologies), and one-color gene expression was performed according to the manufacturer’s protocol. Labeled cRNA was synthesized from 100 ng total RNA using the Low RNA Input Linear Amplification kit (Agilent Technologies) in the presence of cyanine 3-cytosine triphosphate (CTP; PerkinElmer, Boston, MA, USA). Images generated by the Agilent scanner and Feature Extraction 10.5 software (Agilent Technologies) were used to obtain the microarray raw data. Qlucore Omics Explorer software (QOE) (http://www.qlucore.com/; Qlucore, Lund, Sweden) was used to analyze the microarray data.

Callegari E., Domenicali M., Shankaraiah R.C., D’Abundo L., Guerriero P., Giannone F., Baldassarre M., Bassi C., Elamin B.K., Zagatti B., Ferracin M., Fornari F., Altavilla G., Blandamura S., Silini E.M., Gramantieri L., Sabbioni S, & Negrini M. (2018). MicroRNA-Based Prophylaxis in a Mouse Model of Cirrhosis and Liver Cancer. Molecular Therapy. Nucleic Acids, 14, 239-250.

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One-color Gene Expression Microarray Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNAs were hybridized on Agilent Whole Mouse Gene Expression Microarray (G4852A, 8x60K; Agilent Technologies, Palo Alto, CA, USA), and one-color gene expression was performed according to the manufacturer’s protocol. Labeled cRNA was synthesized from 100 ng of total RNA using the Low RNA Input Linear Amplification kit (Agilent Technologies) in the presence of cyanine 3-cytosine triphosphate (CTP) (Perkin-Elmer Life Sciences, Boston, MA, USA). Images generated by Agilent scanner and Feature Extraction 10.5 software (Agilent Technologies) were used to obtain the microarray raw data. Qlucore Omics Explorer software (QOE) (https://www.qlucore.com/) (Qlucore AB, Lund, Sweden) was used to analyze microarray data.

Callegari E., D’Abundo L., Guerriero P., Simioni C., Elamin B.K., Russo M., Cani A., Bassi C., Zagatti B., Giacomelli L., Blandamura S., Moshiri F., Ultimo S., Frassoldati A., Altavilla G., Gramantieri L., Neri L.M., Sabbioni S, & Negrini M. (2018). miR-199a-3p Modulates MTOR and PAK4 Pathways and Inhibits Tumor Growth in a Hepatocellular Carcinoma Transgenic Mouse Model. Molecular Therapy. Nucleic Acids, 11, 485-493.

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