Prolong gold anti fade reagent with 4

Manufactured by Thermo Fisher Scientific

Sourced in Canada

ProLong Gold anti-fade reagent with 4' is a laboratory product designed to preserve and protect fluorescent signals in microscopy samples. It serves as an anti-fade agent, helping to maintain the intensity and clarity of fluorescent markers over time.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Dmem cell culture media, by Thermo Fisher Scientific (1 mentions) Miscript sybr green pcr kit, by Qiagen (1 mentions) Complete edta free protease inhibitor cocktail, by Roche (1 mentions) Miscript 2 rt kit, by Qiagen (1 mentions) Mirneasy mini kit, by Qiagen (1 mentions) Perfectin transfection reagent, by Genlantis (1 mentions) Miscript pcr starter kit, by Qiagen (1 mentions) Fibronectin, by Merck Group (1 mentions) Biotinylated rabbit anti mouse antibody, by Agilent Technologies (1 mentions)

Spelling variants of this product

ProLong Gold (1530 citations) ProLong Gold reagent (65 citations) Anti-fade prolong gold (18 citations) Anti-fade gold (18 citations) ProLong anti-fade reagents (13 citations)

2 protocols using prolong gold anti fade reagent with 4

Comprehensive Molecular Biology Techniques

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Complete EDTA-free protease inhibitor cocktail (Roche Diagnostics (Laval, Quebec, Canada); ProLong Gold anti-fade reagent with 4,6-diamidino-2-phenylindole (DAPI), SlowFade Gold reagent mounting media, cell culture media DMEM, RPMI 1640, and fetal bovine serum (FBS) from Invitrogen (Carlsbad, CA) were purchased from the indicated suppliers.
Lipofectamine 2000 and Lipofectamine RNAiMAX were purchased from Invitrogen (Carlsbad, CA); Per-Fectin transfection reagent was from Genlantis (San Diego, CA).
miRIDIAN microRNA mimics including human hsa-miR-500a-5p, hsa-miR-34c-3p, hsa-miR-93-3p, hsa-miR-381-3p; miRIDIAN microRNA Mimic Negative Control #1 and miRIDIAN microRNA mimic mouse mmu-miR-344-3p; miRIDIAN microRNA inhibitors including human hsa-miR-500a-5p-Hairpin Inhibitor, hsa-miR-34c-3p-Hairpin Inhibitor, hsa-miR-93-3p-Hairpin Inhibitor and hsa-miR-381-3p-Hairpin Inhibitor were purchased from GE Healthcare Dharmacon Inc. (Lafayette, CO).
MGC human PEX2 (Clone ID: 3347824), PEX7 (Clone ID: 5176358), PEX11B (Clone ID: 3866690), and PEX13 (Clone ID: 6285875) sequence-verified full-length cDNA clones were purchased from GE Healthcare Dharmacon Inc. (Lafayette, CO).
Reagents for purification and quantitation of miRNAs including MiRNeasy Mini kit, miScript PCR Starter kit, miScript II RT kit, and miScript SYBR Green PCR kit were purchased from Qiagen (Toronto, ON).

Xu Z., Asahchop E.L., Branton W.G., Gelman B.B., Power C, & Hobman T.C. (2017). MicroRNAs upregulated during HIV infection target peroxisome biogenesis factors: Implications for virus biology, disease mechanisms and neuropathology. PLoS Pathogens, 13(6), e1006360.

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Antibody-based Protein Detection Methods

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Antibodies were used for Western immunoblots, indirect immunofluorescence microscopy, and immunohistochemistry as per manufacturer's instructions: paxillin mouse antibody (P13520; Transduction Laboratories, San Jose, CA), FLI-1 rabbit antibody (15289; Abcam, Cambridge, MA), zyxin rabbit antibody (B71; Beckerle Lab), α5 integrin (610633; BD Bioscience, San Jose, CA), β-actin AC-74 mouse antibody and vinculin V9131 mouse antibody (Sigma-Aldrich, St. Louis, MO), α-tubulin mouse antibody (12G10; Developmental Studies Hybridoma Bank, Iowa City, IA), and CD99 (clone 0-13 mouse antibody; 620-01; Signet, Cambridge, UK). Secondary antibodies were horseradish peroxidase (HRP)–conjugated antibodies for immunoblots (GE Healthcare, Pittsburgh, PA), the Alexa Fluor antibodies and phalloidin for microscopy (Molecular Probes/Invitrogen, Grand Island, NY), and biotinylated rabbit anti-mouse antibody (E0413; Dako, Carpinteria, CA) for immunohistochemistry. Fibronectin to coat coverslips and Mowiol to mount coverslips (Sigma-Aldrich) and Vybrant Cell-Labeling solutions DiI and DiO (Molecular Probes/Invitrogen) were used as per manufacturer's instructions. d-Luciferin (Luck; Gold Biotech, St. Louis, MO) was injected for bioluminescence detection. Prolong Gold anti-fade reagent with 4′,6-diamidino-2-phenylindole (DAPI; P-36931; Invitrogen/Life Technologies) was used for mounting lung sections.

Chaturvedi A., Hoffman L.M., Jensen C.C., Lin Y.C., Grossmann A.H., Randall R.L., Lessnick S.L., Welm A.L, & Beckerle M.C. (2014). Molecular dissection of the mechanism by which EWS/FLI expression compromises actin cytoskeletal integrity and cell adhesion in Ewing sarcoma. Molecular Biology of the Cell, 25(18), 2695-2709.

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