GA3 (G7645) (Sigma-Aldrich, Saint-Quentin-Fallavier, France) diluted with 96° ethanol and stored at −6 °C as the stock solution; dithiothreitol (DTT) (A1101) (Appli-Chem, Darmstadt, Germany) diluted with cell medium and was used once; ER-Tracker Red (BODIPY™ TR Glibenclamide) (E34250) (Thermo Fisher Scientific, Bucharest, Romania, Waltham, MA, USA); rabbit polyclonal antibodies specific to GRP78 (G8918) (Sigma-Aldrich, St. Louis, MI, USA), (PA5-22967) (Thermo Fisher Scientific); LC3B (ab51520) (Abcam, Cambridge, UK), filaggrin (ab81468) (Abcam); mouse monoclonal antibodies specific to involucrin (I9018) (Sigma-Aldrich); Alexa Fluor-488-conjugated rabbit anti-IgG antibodies (ab150077) (Abcam); Alexa Fluor-488-conjugated mouse anti-IgG antibodies (A-11001) (Thermo Fisher Scientific).
Ga3 g7645
Manufactured by Merck Group
Sourced in France
GA3 (G7645) is a laboratory reagent produced by Merck Group. It is a plant growth regulator that can be used in various research applications. The core function of GA3 is to stimulate plant growth and development. Further details on its specific intended uses are not available.
Automatically generated - may contain errors
2 protocols using ga3 g7645
1
ER Stress and Autophagy Evaluation
2
Exogenous Plant Hormone Effects on Grape Adventitious Root Formation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Exogenous plant growth regulators (PGRs) were used to investigate the effects of different hormones on grape ARF. Exogenous auxin (indole butyric acid, IBA, I5386, Sigma-Aldrich) and N-1-naphthylphthalamic acid (NPA, PESTANAL ® , 33371, Sigma-Aldrich), a polar auxin transport inhibitor, were utilized to investigate the key functions of auxin in ARF. Cytokinin (6-benzaminopurine, 6-BA, A8170, Solarbio), ethephon (ET, E8021, Solarbio), abscisic acid (ABA, A8060, Solarbio), gibberellin acid 3 (GA 3 , G7645, Sigma-Aldrich), jasmonic acid methyl ester (MeJA, M8640, Solarbio), 2,4-epibrassinolide (EBR, IE0110, Solarbio), were rst prepared into 10 mg•mL -1 stock solutions using DMSO or NaOH. At the time of exogenous hormone treatment, the stock solution was added to a warm growth medium before pouring into plates to provide a nal concentration.
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