Hs crp

All blood samples were immediately obtained at 08:00 after overnight. Enzyme-linked immunosorbent assay was used to detect the high-sensitivity C-reactive protein (HS-CRP) (Lot 78034031, Bender Med Systems GmbH, Austria; minimum detection limit: 3 pg/ml; intra-assay CVs: 6.9%; inter-assay CVs: 13.1%), matrix metalloproteinase 2 (MMP2) (Lot 303216, R&D, USA; minimum detection limit: 0.047 ng/ml; intra-assay CVs: 5.6%; inter-assay CVs: 7.4%), soluble advanced glycation end products (sRAGE) (Lot 303510, R&D, USA; minimum detection limit: 4.12 pg/ml; intra-assay CVs: 5.7%; inter-assay CVs: 7.7%), and fractalkine (Lot 301156, R&D, USA; minimum detection limit: 0.018 ng/ml; intra-assay CVs: 2.6%; inter-assay CVs: 6.6%). The concentrations of serum AGE-peptides (AGE-P) were measured by flow injection assay (FIA) (24 (link)).
The subject was consumed a standardized breakfast (100 g steamed bread). Venous blood were sampled before and after breakfast, and fasting blood glucose (FBG), HbA1c, total cholesterol (TC), total triglyceride (TG), HDL, LDL, and postprandial blood glucose (PBG) were measured. The glomerular filtration rate (GFR) was estimated using the equation recommended by the National Kidney Foundation in the Modified Diet in Renal Disease (25 (link)).

Sleep quality was examined with the Pittsburgh Sleep Quality Index (PSQI), comprising 19 items that identify seven core elements of sleep: sleep quality, how long subjects take to fall asleep, percentage of time in bed, sleep duration, sleep disturbances, medications for sleep, and daytime dysfunction. Global scores of ≥5 indicate poorsleep and <5 good sleep with a sensitivity of 98.7 and specificity of 84.4 for the diagnosis of sleep quality. Total cholesterol (TC), triglycerides (TG), and high-density lipoprotein (HDL) were examined with a one-step enzymatic method (Erba Mannheim, London, UK) with a minimum detection limit of 0.1 mg/L. Low-density lipoprotein (LDL) was evaluated with the Friedewald equation. An Enzyme Linked Immunosorbent Assay (ELISA) was used to determine plasma levels of IL-6, IL-1β, TNF-α, and hsCRP(ThermoFisher Scientific, Waltham, MA, USA), on a microplate reader (Biotek Instruments Inc., Winooski, VT, USA). Diagnostic sensitivities of the kits were <1.0 pg/mL, 0.35 pg/mL, 1.75 pg/mL, and 9.38 pg/mL for IL-6, IL-1β, TNF-α, and hsCRP, respectively. The coefficients of variation for inter- and intra-assay analyses were less than 5% for all these assays.