Q exactive hesi orbitrap

Manufactured by Thermo Fisher Scientific

The Q Exactive HESI-Orbitrap is a high-resolution mass spectrometer that combines the high mass accuracy and resolving power of the Orbitrap mass analyzer with the versatility of the Heated Electrospray Ionization (HESI) source. It is designed to provide sensitive and reliable detection of a wide range of analytes in various applications.

Automatically generated - may contain errors

Lab products found in correlation

Nano hplc easy nlc 1000, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Q Exactive (1165 citations) Q Exactive Orbitrap (257 citations) Orbitrap Exactive (13 citations)

2 protocols using q exactive hesi orbitrap

Proteomic Analysis of Trypanosoma cruzi EVs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Purified EVs (20 μg) were diluted in 50 mM ammonium carbonate. Mass spectrometry analysis was carried out at Centro de Estudios Químicos y Biológicos por Espectrometría de Masa (CEQUIBIEM), Argentina, in a Q Exactive HESI-Orbitrap coupled to a nano HPLC Easy-nLC 1000 (Thermo Scientific). MS/MS data were used to search the all the available Trypanosoma cruzi databases at Tritrypdb (version 30) [11 (link)].

Caeiro L.D., Alba-Soto C.D., Rizzi M., Solana M.E., Rodriguez G., Chidichimo A.M., Rodriguez M.E., Sánchez D.O., Levy G.V, & Tekiel V. (2018). The protein family TcTASV-C is a novel Trypanosoma cruzi virulence factor secreted in extracellular vesicles by trypomastigotes and highly expressed in bloodstream forms. PLoS Neglected Tropical Diseases, 12(5), e0006475.

+ Open protocol

+ Expand

Proteomic Analysis of T. cruzi

Check if the same lab product or an alternative is used in the 5 most similar protocols

After 10% SDS-PAGE of epimastigotes protein extracts, the section of the gel between 70 kDa and 100 kDa was manually cut and preserved in an eppendorf tube at -80°C until delivery for mass spectrometry analysis. Mass spectrometry analysis was carried out at Centro de Estudios Químicos y Biológicos por Espectrometría de Masa (CEQUIBIEM), Argentina, in a Q Exactive HESI-Orbitrap coupled to a nano HPLC Easy-nLC 1000 (Thermo Scientific). Resulting reads were matched to the T. cruzi proteome available at the UNIPROT proteome database.

Sternlieb T., Schoijet A.C., Genta P.D., Vilchez Larrea S.C, & Alonso G.D. (2021). An AMP-activated protein kinase complex with two distinctive alpha subunits is involved in nutritional stress responses in Trypanosoma cruzi. PLoS Neglected Tropical Diseases, 15(5), e0009435.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!