Carboxymethylcellulose cmc solution

Experimental periodontitis was induced as described previously (27 (link)). In brief, mice were treated with trimethoprim (0.16 mg/mL) and sulfamethoxazole (0.8 mg/mL) solution (Resprim; Teva Pharmaceutical Industries) in the drinking water for 10 days, followed by 3 days without antibiotics. 1 × 10⁹ colony-forming units of P. gingivalis 53977 were introduced via oral gavage, three times at 2-day intervals in 200 µL of 2% (wt/vol) carboxymethylcellulose (CMC) solution (Sigma). Control mice were treated with CMC 2% vehicle alone. The Pg + RvD2 group was treated with three doses of 0.5 µg RvD2 (Cayman Chemical) in a total volume of 150 µl of sterile saline solution intraperitoneally (i.p.) followed by six intraperitoneal doses of 0.1 µg of RvD2 over the next 2 weeks (Figure 1A). The Pg group received 150 µl of sterile saline solution i.p. in the same regimen as the Pg + RvD2. Control mice received 3 oral gavages of 2% CMC 2 days a part. 6 weeks later, mice were euthanized, and the hemi-maxillae were harvested and scanned using micro-computed tomography (μCT) (μCT 40, Scanco Medical, Switzerland) for alveolar bone loss quantification.