Alexa fluor 555 conjugated goat anti rabbit antibody

Manufactured by Thermo Fisher Scientific

Sourced in United States

Alexa Fluor 555-conjugated goat anti-rabbit antibody is a secondary antibody that binds to rabbit primary antibodies. It is conjugated with the Alexa Fluor 555 fluorescent dye, which can be detected using appropriate fluorescence detection methods.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor 488 conjugated goat anti mouse antibody, by Thermo Fisher Scientific (3 mentions) Alexa fluor 488 conjugated phalloidin, by Thermo Fisher Scientific (2 mentions) Fv 1 mm, by Olympus (2 mentions) Anti nestin antibody, by Merck Group (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Santa Cruz Biotechnology (2 mentions) Formalin, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Fujifilm (1 mentions) Lsm 510 confocal microscope, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Nb100 304, by Novus Biologicals (1 mentions) Clone jbw301, by Merck Group (1 mentions) Pneumococcus type 2 serum, by SSI Diagnostica (1 mentions) Alexa fluor 488 conjugated goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor 555 conjugated goat anti mouse antibody, by Thermo Fisher Scientific (1 mentions) Prolong diamond antifade mountant, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) A1r confocal microscope, by Nikon (1 mentions) Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions) Leica sp5 confocal microscope, by Leica Microsystems (1 mentions) Lsm 880 confocal laser scanning microscope, by Zeiss (1 mentions)

10 protocols using alexa fluor 555 conjugated goat anti rabbit antibody

Immunofluorescence Analysis of β-Catenin in DP Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human or rat DP cells were seeded in a 12‐well plate on coverslips. The cells were incubated with 0.1% (v/v) DMSO as the vehicle, 1‐ or 5‐μM KY19382, or 100‐μM minoxidil for 48 h. Cultured cells were washed twice with cold PBS and were fixed in 4% paraformaldehyde (Wako, Osaka, Japan) or 10% formalin (Sigma‐Aldrich, St. Louis, USA) for 15 min at room temperature and then were washed with PBS and permeabilized with 0.2% Triton X‐100 for 15 min.
After blocking with 5% BSA in PBS for 30 min at room temperature, the cells were blotted with primary antibody: rabbit anti‐β‐catenin (Abcam Cat# 16051, RRID:AB_443301, 1:50, Cambridge, USA) overnight at 4°C. After washing with PBS, the cells were blotted with Alexa Fluor 488‐conjugated goat anti‐mouse antibody (Thermo Fisher Scientific Cat# A‐11001, RRID:AB_2534069, 1:300, Waltham, USA) or Alexa Fluor 555‐conjugated goat anti‐rabbit antibody (Thermo Fisher Scientific Cat# A‐21428, RRID:AB_2535849, 1:300, Waltham, USA) for 1 h at room temperature and counterstained with DAPI (Sigma‐Aldrich, St. Louis, USA) for 10 min at room temperature. Images were taken using an LSM510 confocal microscope (Carl Zeiss Inc., Germany). The fluorescence intensity was quantified using Zen software V3.1 software (ZEN Digital Imaging for Light Microscopy, RRID:SCR_013672, Germany).

Ryu Y.C., Lee D., Shim J., Park J., Kim Y., Choi S., Bak S.S., Sung Y.K., Lee S, & Choi K. (2021). KY19382, a novel activator of Wnt/β‐catenin signalling, promotes hair regrowth and hair follicle neogenesis. British Journal of Pharmacology, 178(12), 2533-2546.

+ Open protocol

+ Expand

Immunofluorescent Detection of DNA Damage Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

γH2AX and 53BP1 were detected in the PBMCs of healthy donors and CML patients using a mouse monoclonal anti-γH2AX antibody (clone JBW301; Merck Millipore, Darmstadt, Germany) and a polyclonal rabbit anti-53BP1 antibody (NB100-304; Novus Biologicals, Littleton, US), respectively. An Alexa Fluor 488-conjugated goat anti-mouse antibody and an Alexa Fluor 555-conjugated goat anti-rabbit antibody (Thermo Fisher Scientific) were used as previously described [38 (link),39 (link)]. At least 50 PBMCs were analyzed for each measurement.

Popp H.D., Kohl V., Naumann N., Flach J., Brendel S., Kleiner H., Weiss C., Seifarth W., Saussele S., Hofmann W.K, & Fabarius A. (2020). DNA Damage and DNA Damage Response in Chronic Myeloid Leukemia. International Journal of Molecular Sciences, 21(4), 1177.

+ Open protocol

+ Expand

Visualizing Pneumococcus Polysaccharide Capsule

Check if the same lab product or an alternative is used in the 5 most similar protocols

For visualization of the polysaccharide capsule, S. pneumoniae was grown in CY medium supplemented with or without 100 ng⋅μL ATc until OD (600 nm) 0.3, then rediluted into fresh CY medium and grown until OD (600 nm) 0.1. Cells were then harvested by centrifugation, washed, and resuspended in fresh prewarmed CY medium. The 1:1,000 pneumococcus type 2 serum (SSI Diagnostica) was added, and cells were incubated for 5 min on ice. After two washes with fresh CY medium, 1:1,000 of secondary Alexa Fluor 555-conjugated goat anti-rabbit antibody (Invitrogen; A27039) was added, and cells were incubated in ice for 5 min. The stained cells were then washed twice with fresh CY medium and once with ice-cold PBS. The pellet was then resuspended in PBS for imaging using epifluorescence.

Sorg R.A., Gallay C., Van Maele L., Sirard J.C, & Veening J.W. (2020). Synthetic gene-regulatory networks in the opportunistic human pathogen Streptococcus pneumoniae. Proceedings of the National Academy of Sciences of the United States of America, 117(44), 27608-27619.

+ Open protocol

+ Expand

Fluorescent Immunohistochemistry of Pancreas

Check if the same lab product or an alternative is used in the 5 most similar protocols

For fluorescent immunostaining, paraffin pancreas section was de-waxed and rehydrated to distilled water. Antigen retrieval was performed by boiling in microwave for 10 min in 0.01 M citrate buffer (pH 6.0). After cooling, the section was blocked in 10% goat serum plus 1% BSA for 1 h at room temperature. Primary antibody incubation was performed at 4˚C overnight. Corresponding secondary fluorescent antibodies were applied to the section for 1 h at room temperature. Cell nuclei were counterstained with DAPI (Sigma, D9542). Image was taken with a fluorescent microscope (ZISS). For staining against Pax6, a 5 min treatment with cold (-20˚C) methanol was included after antigen retrieval. Primary antibodies used in this study were FITC-conjugated mouse anti-insulin (ebioscience, 53-9769-82, 1:200), rabbit anti-glucagon (Abcam, ab93527, 1:1000), rabbit anti Pax6 (Biolegend, 90130 1:300). Secondary antibodies used in this study was Alexa Fluor 555-conjugated goat anti-rabbit antibody (Invitrogen, A27039, 1:400).

Sheng J., Wang L., Tang P.M., Wang H.L., Li J.C., Xu B.H., Xue V.W., Tan R.Z., Jin N., Chan T.F., Huang X.R., Ma R.C, & Lan H.Y. (2021). Smad3 deficiency promotes beta cell proliferation and function in db/db mice via restoring Pax6 expression. Theranostics, 11(6), 2845-2859.

+ Open protocol

+ Expand

Quantification of p75NTR-Positive Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

After 7 days in vitro, the cells were trypsinized and washed with PBS, and recovered by centrifugation at 1200 rpm for 5 min. The cells were resuspended in 1 ml of PBS, fixed and permeabilized for 1 h at 4°C by adding ethanol 100% to a final concentration of 70% ethanol. The cells were then washed and resuspended in PBS + BSA (5 mg/ml) for 30 min at room temperature to block non-specific antigen binding, and they were then stained overnight at 4°C with an anti-p75NTR antibody with shaking (Table 2). After washing for 1 h at room temperature, the primary antibody was detected with a secondary Alexa Fluor 555 conjugated goat anti-rabbit antibody (Invitrogen, Eugene, OR, United States) and the cells were analyzed on a FACScan (Beckman Coulter Gallios, Brea, CA, United States) to identify those positive for p75NTR.

Pereiro X., Ruzafa N., Acera A., Urcola A, & Vecino E. (2020). Optimization of a Method to Isolate and Culture Adult Porcine, Rats and Mice Müller Glia in Order to Study Retinal Diseases. Frontiers in Cellular Neuroscience, 14, 7.

+ Open protocol

+ Expand

Visualizing EZH2 and Nucleolar Protein Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols

To detect the co-localization of endogenous EZH2 and FBL, C4–2 cells were cultured on coverslips for 24 h. Cells were fixed in pre-cooled methanol for 15 min and permeabilized in phosphate-buffered saline (PBS) containing 0.5% Triton X-100 for 10 min at room temperature. Slides were rinsed thrice with PBS and blocked with 5% BSA for 30 min. Then, the slides were incubated with rabbit anti-EZH2 antibody and mouse anti-FBL antibody at 4 °C overnight. Slides were rinsed thrice with PBS, followed by exposure to Alexa Fluor 488 conjugated goat anti-rabbit antibody and Alexa Fluor 555 conjugated goat anti-mouse antibody (all from Invitrogen) for additional 1 h at 37 °C. Cells were co-stained in the dark with DAPI (Invitrogen) and mounted using ProLong Diamond Antifade Mountant (Invitrogen). Immunostained cells were viewed using Nikon A1R confocal microscope.
For observation of localization of EZH2 truncation mutants, C4–2 cells were transfected with either GFP-full length EZH2 or GFP-truncated EZH2 plasmids. At 48 h post-transfection, the IF assay was performed as described above except for the use of mouse anti-GFP antibody and rabbit anti-FBL/anti-NOP56 antibody as primary antibodies, and Alexa Fluor 488 conjugated goat anti-mouse antibody and Alexa Fluor 555 conjugated goat anti-rabbit antibody (all from Invitrogen) as secondary antibodies.

Yi Y., Li Y., Meng Q., Li Q., Li F., Lu B., Shen J., Fazli L., Zhao D., Li C., Jiang W., Wang R., Liu Q., Szczepanski A., Li Q., Qin W., Weiner A.B., Lotan T.L., Ji Z., Kalantry S., Wang L., Schaeffer E.M., Niu H., Dong X., Zhao W., Chen K, & Cao Q. (2021). A PRC2-independent function for EZH2 in regulating rRNA 2′-O methylation and IRES-dependent translation. Nature cell biology, 23(4), 341-354.

+ Open protocol

+ Expand

Retinal Pigment Epithelium Flat Mount

Check if the same lab product or an alternative is used in the 5 most similar protocols

After enucleation, mouse eyes were punctured with a fine needle and immersion fixed in ice-cold 10% neutral-buffered formalin (NBF) for 10 minutes. The anterior segment was removed, and the neural retina was separated from the RPE-choroid-sclera. Six radial cuts were made toward the optic nerve to flatten the posterior eyecup. After total fixation of 30 minutes in NBF, the tissue was washed three times with PBS, incubated with PBST (PBS with 0.1% Tween-20) for 20 minutes, and blocked with 5% goat serum in PBST for 30 minutes. For phalloidin staining, tissues were incubated with Alexa Fluor 488-phalloidin (1:300; Invitrogen) in blocking solution overnight at 4°C; for double staining, tissues were incubated with ZO-1 antibody (1:300 dilution) in blocking solution overnight at 4°C, washed with PBST, and incubated with Alexa Fluor 555–conjugated goat anti-rabbit antibody (1:200; Invitrogen) and Alexa Fluor 488 phalloidin (1:300; Invitrogen) for 2 hours. Nuclei were stained by incubating with 4′,6-diamidino-2-phenylindole (DAPI) for 20 minutes. The whole RPE-choroid-sclera tissue was then flat mounted, with RPE side up, onto slides and cover-slipped for imaging. Fluorescent images were captured with a Leica SP5 confocal microscope (Leica Microsystems, Buffalo Grove, IL, USA), and identical imaging parameters were applied to both WT and mutant RPE flat mounts.

Ji X., Liu Y., Hurd R., Wang J., Fitzmaurice B., Nishina P.M, & Chang B. (2016). Retinal Pigment Epithelium Atrophy 1 (rpea1): A New Mouse Model With Retinal Detachment Caused by a Disruption of Protein Kinase C, θ. Investigative Ophthalmology & Visual Science, 57(3), 877-888.

+ Open protocol

+ Expand

Immunofluorescence microscopy of Nl Gr23a in labium

Check if the same lab product or an alternative is used in the 5 most similar protocols

The tissue slices were processed for immunofluorescence microscopy as previously described [34 (link)]. The labium of a one-day-old brachypterous female adult was removed, washed with 70% ethanol, and fixed in 4% paraformaldehyde at room temperature for 2 h. After fixation, the labium was embedded in Tissue-Tek O.C.T. compound (Sakura Finetek, Tokyo, Japan) and frozen at −20 °C. The embedded specimens were mounted on an object holder and cryosectioned using a Leica CM1950 cryostat (Leica Biosystems, Wetzlar, Germany) at 6 µm thickness. The cryosections were placed on adhesion glass slides (CITOGLAS, Jiangsu, China) and air dried at room temperature for 4 h. HPLC purified rabbit antibody against NlGr23a peptide CTLESRKVLSIKSKN (8 µg/mL) was used as the primary antibody, and Alexa Fluor 555^®-conjugated goat anti-rabbit antibody (1:400; Invitrogen, Carlsbad, CA, USA) was used as the secondary antibody. Prior to observation, the samples were stained with Hoechst 33342 (Invitrogen, Carlsbad, CA, USA) and washed two times with PBS. The tissue slices were visualized with a Zeiss LSM 880 laser scanning confocal microscope (Carl Zeiss, Oberkochen, Germany).

Kang K., Zhang M., Yue L., Chen W., Dai Y., Lin K., Liu K., Lv J., Guan Z., Xiao S, & Zhang W. (2023). Oxalic Acid Inhibits Feeding Behavior of the Brown Planthopper via Binding to Gustatory Receptor Gr23a. Cells, 12(5), 771.

+ Open protocol

+ Expand

Immunofluorescence Staining of Nestin and F-Actin

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sliced tissues on coverslips were washed three times with phosphate-buffered saline (PBS), fixed with 4% paraformaldehyde in PBS for 10 min, and permeabilized with 0.1% Triton X-100 in PBS for 5 min at room temperature. After washing three times with PBS, sections were blocked with 1% bovine serum albumin (BSA) for 1 h at room temperature. Sections were incubated with anti-nestin antibody (N5413, Sigma-Aldrich, St. Louis, MO) (diluted in blocking solution (1% BSA in PBS)) for 1 h at room temperature in a shading box. Subsequently, the tissue sections were washed three times with PBS and incubated with secondary antibody, Alexa Fluor 555-conjugated rabbit anti-goat antibody (Invitrogen, Grand Island, NY) for 1 h at room temperature. Alexa Fluor 488-conjugated phalloidin (Invitrogen, Grand Island, NY) was used for F-actin staining. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI, Santa Cruz Biotechnologies, Santa Cruz, CA). Images were captured using a confocal microscope (Olympus, FV-1 mm).

Lee S.H., Cho H.G., Song J.S., Chun K.C, & Chun C.H. (2020). Quantitative assessment of neural elements in a rat model using nerve growth factor after remnant-preserving anterior cruciate ligament reconstruction: a histological and immunofluorescence pilot study. Journal of Orthopaedic Surgery and Research, 15, 277.

+ Open protocol

+ Expand

Immunofluorescence Staining of Nestin

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells grown on coverslips were washed three times with phosphate-buffered saline (PBS), fixed with 4% paraformaldehyde in PBS for 10 min, washed three times with PBS, and then permeabilized with 0.1% Triton X-100 in PBS for 5 min at room temperature. After washing three times in PBS, cells were blocked with 1% bovine serum albumin (BSA) for 1 h at room temperature. Incubation with anti-Nestin antibody (Sigma, St. Louis, MO) was performed in blocking solution (1% BSA in PBS) for 1 h at room temperature in a light-proof box. Specimens were washed three times with PBS and incubated for 1 h at room temperature with Alexa Fluor 555-conjugated rabbit anti-goat antibody (Invitrogen, Grand Island, NY).
For F-actin staining, Alexa Fluor 488-conjugated phalloidin (Invitrogen, Grand Island, NY) was used. Nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI, Santa Cruz Biotechnologies, Santa Cruz, CA). A confocal microscope (Olympus, FV-1mm) was used for capturing images.

Chun K.C., Lee S.H., Kim J.W., Jin E.J., Kim K.M, & Chun C.H. (2017). Immunohistochemical and immunocytochemical study of mechanoreceptors in anterior cruciate ligament reconstruction with the remnant-preserving technique using Achilles tendon allografts. Journal of Orthopaedic Surgery and Research, 12, 93.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!