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4 protocols using ceftazidime

1

Antibiotic Susceptibility Profiling of MRSA

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The antibiotic susceptibility profiles for the VT-MRSA isolates were determined using the MIC technique via the microdilution method according to the CLSI 2013 [32 ], as described in the previous section. MIC tests were performed for ampicillin (Sigma-Aldrich), ceftazidime (Pfizer; México DF, México), ceftriaxone (Roche; Mexico DF, México), ciprofloxacin (Bayer; Levenkusen Westfalia, Germany), erythromycin (MP Biomedicals; Solon, OH, USA), kanamycin (Sigma-Aldrich), meropenem (Astra Zeneca; México DF, Mexico), rifampicin (MP Biomedicals), gentamicin (MP Biomedicals), and trimethoprim-sulfamethoxazole (Sigma-Aldrich).
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2

Antibiotic Susceptibility Testing Protocol

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Imipenem, meropenem, ceftazidime, and tigecycline was purchased from Pfizer. MH agar was purchased from OXOID. Primers were synthesized by Sangon Biotech Shanghai Co. Ltd. Premix Taq was purchased from Takara Biotech Dalian Co. Ltd. Xba I restriction enzymes were purchased from Thermo.
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3

Preparation and Administration of Delafloxacin and Ceftazidime

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The intravenous formulation of delafloxacin (Melinta Therapeutics, Morristown, NJ, USA) was used in the study. Reconstituted solutions were stable for up to 24 h. Liquid suspensions were prepared fresh in 0.9% saline irrigation (SI) daily and stored at 2 to 8°C. The volume of subcutaneous (SC) administrations was 0.2 ml.
The parenteral formulation of ceftazidime (Hospira, Lake Forest, IL, USA) was used in the study and was stored under refrigeration at 2 to 8°C.
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4

Perioperative Antimicrobial Prophylaxis Protocol

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Ceftazidime (Tazicef, Hospira, Inc., Lake Forest, IL) was injected IV at 150 mg/kg on the day of surgery and continued through the study as long as tether once daily. Fluconazole (NorthStar Rx, Memphis, TN) was administered orally at a 50-mg flat dose per animal starting on day –5 and then once daily. Acyclovir (AuroMedics Pharma, Windsor, NJ) was delivered IV at 10 mg/kg starting on day –5, and then once daily.
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