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Ec epiplan apochromat

Manufactured by Zeiss

The EC Epiplan-Apochromat is a high-performance microscope objective lens designed by Zeiss. It provides superior optical performance for a wide range of microscopy applications. The lens is built with an apochromatic design, which minimizes chromatic aberrations and delivers high-quality, distortion-free images.

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4 protocols using ec epiplan apochromat

1

Angle-Resolved Spectroscopy of Optical Samples

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A customized optical microscope operating in dark-field (DF) mode was used for the micro-spectroscopic investigation of the fabricated samples. A halogen lamp was used as a light source using a 100 × objective (EC Epiplan-APOCHROMAT, Zeiss) with a numerical aperture of numerical aperture (NA)=0.95. The scattered light was collected in a confocal configuration using a 50-μm core optical fiber (Avantes, Leatherhead, Surrey, UK) and analyzed using a spectrometer (AvaSpec-HS2048, Avantes). The spatial resolution of the collected spectra was ~1 μm. Larger-area spectroscopic characterizations were performed using a 600-μm optical fiber, permitting a spatial resolution of ~25 μm. Furthermore, the angle-resolved scattering was measured using a home-built optical goniometric setup24 (link). Light from a deuterium-halogen lamp (DH-2000, Ocean Optics, Dunedin, FL, USA) was collimated to form a 1-mm-wide parallel incident beam that illuminated the sample at a fixed angle. The scattered light was detected at multiple angles with an angular resolution of 1° and coupled into an optical fiber connected to the spectrometer (QE65000 Ocean Optics). All spectra were referenced to a white Lambertian reflectance standard (Spectralon, ≈99% reflectance).
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2

Polarized Optical Microscopy of CNC Films

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Polarized optical microscopy (Zeiss Axio Scope A1) images of the CNC films were taken using a 20× objective (Zeiss EC Epiplan APOCHROMAT, NA = 0.3). The light reflected by the CNC and CNC–EC films passed through a quarter‐wave plate and an orientable linearly polarizing filter, which can together filter either the left‐ or the right‐circularly polarized light reflected by the sample (denoted LCP and RCP, respectively). A beamsplitter allowed the light to be directed at a CMOS camera (UI‐3580LE, IDS) and a fiber‐coupled spectrometer (Avantes AvaSpec HS2048), which collects light from a defined region of the microscope field of view. A 600 µm core optical fiber (Thorlabs FC‐UV600‐2‐SR) was used for measuring the reflectance from the films via a magnifying lens (Thorlabs AC254‐050‐A). As a result, spectra were acquired with a spot size of ≈100 µm. The spectra were normalized to the reflection from a silver mirror (Thorlabs PF10‐03‐P01) in one polarization channel (LCP), such that a perfectly aligned cholesteric sample would reflect 100% LCP intensity.
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3

Microspectroscopy of Individual Scales

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A customized Zeiss Axio microscope with a spot diameter of ≈25 μm in bright-field reflection and transmission mode with a halogen lamp in Koehler illumination was used for the microspectroscopic analysis of individual scales. Unpolarized light from the halogen lamp was illuminated via a 10× objective (EC Epiplan-Apochromat, Zeiss) with a numerical aperture (NA) of 0.3 for reflection measurement. A condenser with a NA of 0.25 was used for transmitted light. The transmitted and reflected light was collected with a spectrometer (AvaSpec-HS2048, Avantes) through a 200-μm core optical fiber (Avantes) mounted in confocal configuration. Absorption was then derived by applying A = 1 − R − T of the integrating sphere measurements.
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4

Imaging Techniques for Nanoscale Characterization

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Optical micrograph in Fig. 1 was taken using epi-illumination through a 10× objective lens (Zeiss EC Epiplan-Apochromat) using a commercial microscope (Zeiss Axiophot) and camera (Zeiss AxioCamERc5s). Scanning electron micrographs were collected using an FEI Scios FIB/SEM instrument using 30 keV electrons, 6144  ×  4376 pixels, a 32  μ m aperture, a nominal magnification of 5 k × and a dwell time of 5  μ s, a working distance of 7 mm; the secondary electrons used to create the image were collected using an Everhart–Thornley detector mounted near the column. For each experiment, the two micrographs were aligned with each other by cross correlation to remove drift, cropped to 80  μ m wide and binned 4× to create the image for the figure. Beam current was measured using a Faraday cup mounted on the specimen stage. For the trajectory calculations, after drift correction on the complete micrographs, the selected region of the image stack was subdivided into individual 128  ×  128  ×  2 pixel stacks, and the movement in each was measured by cross correlation. These were then used to generate the vector plots in the figure. Each vector magnitude was multiplied by four to make them easier to see in the plot. Transmission electron micrograph in Fig. 1 was taken using 300 keV electrons on a Gatan K2 direct electron detector operated in super-resolution counting mode in a FEI Titan Krios.
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