The plasma samples of the participants were collected on the same day that the clinical and cognitive evaluations were performed and stored at −80 °C until they were assayed. The BDNF levels were measured with a human BDNF ELISA Kit, which was performed as instructed by the manufacturer (R&D Systems, Minneapolis, MN, USA).
Human bdnf elisa kit
Manufactured by R&D Systems
Sourced in United States
The Human BDNF ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human brain-derived neurotrophic factor (BDNF) in cell culture supernatants, serum, and plasma samples.
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4 protocols using human bdnf elisa kit
1
Plasma BDNF Quantification Protocol
2
BDNF Quantification in MSC Secretome
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Enzyme-linked immunosorbent assay (ELISA) was performed to quantitate BDNF in the culture supernatant. Naïve MSCs and BDNF-eMSCs were seeded at a density of 1 × 105 cells per 12-well plate (Corning, Corning, NY, USA) with 1 mL complete media. Removed cell debris supernatant was obtained after 48 h incubation. BDNF concentrations were analyzed using a Human BDNF ELISA kit (R&D Systems, Minneapolis, MN, USA). The absorbance of the samples at 450 nm was measured using a SpectraMAX 190 (Molecular Devices, San Jose, CA, USA) microplate reader.
3
Quantifying Neurotrophin Levels in Serum
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Serum BDNF, NGF, and GDNF levels were measured using sandwich enzyme-linked immunosorbent assays (ELISAs). For BDNF, we used the Human BDNF ELISA Kit (#DBD00; R&D Systems, Minneapolis, MN, USA); for NGF we used the NGF sandwich ELISA Kit (#CYT304; ChemiKine, Temecula, CA, USA), and for GDNF we used the GDNF Human ELISA Kit (#ab100525; Abcam, Cambridge, MA, USA). Fluorescence was measured at 450 nm with a microplate reader (Emax; Molecular Devices, Sunnyvale, CA, USA).
4
Fasting BDNF and MMP-9 Biomarker Assessment
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Fasting BDNF and MMP-9 tests were performed using native blood samples collected by the non-contact method. Blood serum concentrations of BDNF were measured by ELISA according to the Human BDNF ELISA Kit user manual (R&D Systems), and the concentrations of MMP-9 in serum were tested using a Human MMP-9 ELISA Kit (Biorbyt).
The tests were performed twice in each group, at the beginning and at the end of the experiment.
The tests were performed twice in each group, at the beginning and at the end of the experiment.
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