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Human dpp 4

Manufactured by Merck Group
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Sourced in United States, United Kingdom

Human DPP-IV is a lab equipment product used to measure the activity of the dipeptidyl peptidase-IV enzyme in human samples. It provides a quantitative assessment of DPP-IV levels, which is a key enzyme involved in the regulation of various physiological processes.

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Lab products found in correlation

P nitrophenyl α d glucopyranoside, by Merck Group (1 mentions) α glucosidase from saccharomyces cerevisiae, by Merck Group (1 mentions) Tris hcl buffer, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Acetonitrile acn, by Merck Group (1 mentions) Tris hcl, by Merck Group (1 mentions) Hepes, by Solarbio (1 mentions) Gly pro amc, by AAT Bioquest (1 mentions) Pepsin, by Merck Group (1 mentions) Diprotin a, by Merck Group (1 mentions) Chlorogenic acid, by Merck Group (1 mentions) Phosphoramidon, by Merck Group (1 mentions) Sitagliptin, by Merck Group (1 mentions)

Close spelling variants of this product

Human recombinant DPP-IV Human recombinant DPP-IV enzyme DPP-IV

4 protocols using human dpp 4

1

Inhibition Assay of DPP-IV and α-Glucosidase

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Human DPP-IV (Sigma-Aldrich, Cat. No. D4943), Gly-Pro-p-Nitroanilide Hydrochloride (GPPN) (Sigma-Aldrich, Cat. No. G0513), Tris-HCl Buffer (Sigma-Aldrich, Cat. No. T3253), α-glucosidase from Saccharomyces cerevisiae (Sigma-Aldrich, Cat. No. G5003), p-nitrophenyl-α-D-glucopyranoside (Sigma-Aldrich, Cat. No. N1377) and the other chemicals were purchased from Merck. Sitagliptin and acarbose, which used as reference drugs, were purchased from PT Kimia Farma Tbk., Indonesia, and PT Pharos Tbk., Indonesia, respectively.
Aulifa D.L., Adnyana I.K., Sukrasno S, & Levita J. (2022). Inhibitory activity of xanthoangelol isolated from Ashitaba (Angelica keiskei Koidzumi) towards α-glucosidase and dipeptidyl peptidase-IV: in silico and in vitro studies. Heliyon, 8(5), e09501.
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2

Enzymatic Hydrolysis of Oat Protein

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The oats were provided by Lvba Grain and Oil Group Co., Ltd. (Zhangjiakou, Hebei, China). Flavourzyme, papain, neutrase, and alcalase were purchased from Pangbo Biological Engineering Co., Ltd. (Nanning, Guangxi, China). The 2,4,6-trinitrobenzene sulfonic acid (TNBS) was purchased from Huaxia Reagent Co., Ltd. (Chengdu, Sichuan, China). Pepsin (from porcine gastric mucosa, 3200 U/mg), pancreatic (from porcine pancreas, 3 × USP specifications), Tris-HCl, human DPP-IV, acetonitrile (ACN), and formic acid (FA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetic acid was purchased from Acro Biosystems Co., Ltd. (Beijing, China). L-Leu and HEPES were purchased from Solarbio Technology Co., Ltd. (Beijing, China). Gly-Pro-AMC was purchased from AAT Bioquest (Sunnyvale, CA, USA). All of the other chemicals were of analytical grade.
Wang W., Liu X., Li Y., You H., Yu Z., Wang L., Liu X, & Ding L. (2022). Identification and Characterization of Dipeptidyl Peptidase-IV Inhibitory Peptides from Oat Proteins. Foods, 11(10), 1406.
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3

DPP-IV Inhibition Assay Protocol

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Chlorogenic acid (≥95%), human DPP-IV, Gly-Pro-4-methoxy-β-naphthylamide (≥98%), and Diprotin A (≥97%) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Unnamed chemicals used for reagent preparation were of analytical grade.
Balogun F.O., Naidoo K., Aribisala J.O., Pillay C, & Sabiu S. (2022). Cheminformatics Identification and Validation of Dipeptidyl Peptidase-IV Modulators from Shikimate Pathway-Derived Phenolic Acids towards Interventive Type-2 Diabetes Therapy. Metabolites, 12(10), 937.
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4

Peptidase Inhibition Assay Protocol

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Human PP and PP analogs were purchased from Bachem Ltd. (St. Helens, UK). Human recombinant NEP (EC3.4.24.11) was purchased from R&D Systems (Abingdon, UK). Human DPPIV, the DPPIV inhibitor sitagliptin, and the metallopeptidase inhibitor phosphoramidon, were purchased from Sigma-Aldrich (Dorset, UK).
Cuenco J., Minnion J., Tan T., Scott R., Germain N., Ling Y., Chen R., Ghatei M, & Bloom S. (2017). Degradation Paradigm of the Gut Hormone, Pancreatic Polypeptide, by Hepatic and Renal Peptidases. Endocrinology, 158(6), 1755-1765.
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