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Luna 3 μm silica 2 100

Manufactured by Phenomenex

Luna 3 μm Silica (2) 100 Å is a high-performance liquid chromatography (HPLC) column packing material. It consists of silica particles with a pore size of 100 Angstroms (Å) and a particle size of 3 micrometers (μm). This material is designed for use in HPLC applications.

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3 protocols using luna 3 μm silica 2 100

1

Comprehensive Phospholipid Analysis by HPLC-MS

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MS analysis of phospholipids was performed on an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher). Phospholipids were separated on a normal phase column (Luna 3 μm Silica (2) 100 Å, 150 × 2.0 mm, Phenomenex) at a flow rate of 0.2 ml min−1 on a Dionex Ultimate 3000 HPLC system. The column was maintained at 35 °C. Analysis was performed using gradient solvents (A and B) containing 10 mM ammonium acetate. Solvent A contained propanol:hexane:water (285:215:5, vol/vol/vol) and solvent B contained propanol:hexane:water (285:215:40, vol/vol/vol). All solvents were LC–MS grade. The column was eluted for 0–23 min with a linear gradient of 10–32% B; 23–32 min using a linear gradient of 32–65% B; 32–35 min with a linear gradient of 65–100% B; 35–62 min held at 100% B; 62–64 min with a linear gradient from 100% to 10% B followed by and equilibration from 64 to 80 min at 10% B. The instrument was operated with the electrospray ionization probe in negative polarity mode. Analysis of LC–MS data was performed using software package Compound Discoverer (ThermoFisher) with an in-house generated analysis workflow and oxidized phospholipid database. Lipids were further filtered by retention time and confirmed by fragmentation mass spectrum.
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2

Phospholipid Analysis by LC/MS

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Lipids were extracted using the Folch procedure, and phosphorus was determined by a micro-method as described previously [3 (link)]. Phospholipids were analyzed by LC/MS using a Dionex Ultimate 3000 HPLC system coupled on-line to the Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) using a normal phase column (Luna 3 μm Silica (2) 100 Å, 150 × 2.0 mm, (Phenomenex)) as described previously [3 (link)].
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3

Phospholipid Separation and Analysis

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Phospholipids were separated on a normal phase column (Luna 3 μm Silica (2) 100Å, 150 × 2.0 mm, (Phenomenex)) at a flow rate of 0.2 mL/min on a Dionex Ultimate 3000 HPLC system. The column was maintained at 35°C. The analysis was performed using gradient solvents (A and B) containing 10 mM ammonium acetate and 0.5% triethylamine. Solvent A contained propanol:hexane:water (285:215:5, v/v/v) and solvent B contained propanol:hexane:water (285:215:40, v/v/v). All solvents were LC/MS grade. The column was eluted for 0.5 min isocratically at 25% B, then from 0.5 to 6.5 min with a linear gradient from 25% to 40% solvent B, from 6.5–25 min using a linear gradient of 40–55% solvent B, from 25–38 min with a linear gradient of 55–70% solvent B, from 38–48 min using a linear gradient of 70%–100% solvent B, then isocratically from 48–55 min at 100% solvent B followed by a return to initial conditions from 55–70 min from 100% to 25% B. The column was then equilibrated at 25%B for an additional 5 min.
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