Cell fractionation was performed using a nuclear/cytosol fractionation kit (K266-25; BioVision, Milpitas, CA, USA) according to the manufacturer's instructions. In brief, the cells were lysed and the cell suspension was centrifuged at 15,000g for 5 minutes. The resulting supernatant was then collected as the cytosol fraction. The cytosol-depleted pellet was solubilized in nuclear extraction buffer. Following centrifugation at 15,000g for 10 minutes, the supernatant was collected as the nuclear fraction. Fraction purity was analyzed by immunoblotting. Histone H3 and β-actin were used as nuclear and cytosol-specific markers, respectively.
K266 25
Manufactured by Abcam
Sourced in United States
K266-25 is a lab equipment product. It serves a core function, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using k266 25
1
Subcellular Fractionation and Purification
2
Nuclear and Cytosolic RNA Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HepG2, L02, and A549 (200 w) cell nuclear and cytosolic RNA was extracted using a nuclear/cytosol fractionation kit (BioVision, K266-25). Briefly, the cells were lysed with CEBA-A mix buffer and then centrifuged. The supernatant RNA (cytosol RNA) was extracted with Trizol. The pellet was then washed and lysed with Trizol for nuclear RNA extraction.
3
HDAC Activity Fluorometric Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Extracts of nuclear and cytoplasmic proteins were made from isolated tissues (BioVision K266-25, Waltham, Massachusetts, USA). HDAC enzyme activity was determined using an HDAC Activity Fluorometric Assay Kit (BioVision K330-100, Waltham, Massachusetts, USA) and normalized to protein concentration in each extract.
4
Nuclear-Cytoplasmic Fractionation of PaCa44 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To perform nuclear/cytoplasmic fractionation, 2 × 106 PaCa44 cells were treated with VPA (10 mM) for 48 h. Collected cells were then centrifuged at 600g for 5 min at 4 °C and then lysed, first with CEB-A mix and CEB-B mix for the recovery of cytoplasmic fraction, and finally with NEB-mix for the recovery of nuclear cytoplasmic according to the manufacturer’s instructions (K266-25; Biovision Waltham, MA, USA). Proteins derived from the two different fractions were subsequently analyzed by western blot analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!