2 4 6 trichloro 1 3 5 triazine cyanuric chloride

The liposomes were formulated using specific phospholipids: dipalmitoylphosphatidylcholine (DPPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N [amino (polyethylene glycol)-2000] (DSPE-PEG (2000)-NH₂) are obtained from Avanti Polar Lipids Inc. (Alabaster, AL, USA.). Calcein disodium salt (C₃₀H₂₄N₂Na₂O₁₃), cholesterol, bicinchoninic acid kit, Sephadex G-100, Triton X-100, ammonium ferrothiocyanate (AF) are acquired from Sigma Aldrich (St. Louis, Missouri, USA). Trastuzumab (Herceptin) was purchased from Hoffmann-La Roche Limited (Basel, Switzerland, supplied by Aster Pharmacy, Sharjah, UAE). The 0.2-μm and 0.05-μm polycarbonate membrane filters and filter support were purchased from Whatman PLC (Maidstone, England, U.K.). 2,4,6 trichloro-1,3,5 triazine (cyanuric chloride) was procured from Sigma-Aldrich (St. Louis, MO, US). Perfluoropentane (PFC₅) was acquired from Strem Chemicals (Newburyport, MA, USA).

Dipalmitoylphosphatidyl choline (DPPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N [amino (polyethylene glycol)-2000] (DSPE-PEG (2000)-NH2) were obtained from Avanti Polar Lipids Inc. (Alabaster, AL, USA, supplied by Labco LLC. Dubai, UAE). Cholesterol, calcein disodium salt, L-glutamine, antibiotic solutions (penicillin and streptomycin), trypsin, fetal bovine serum (FBS), RPMI-1640 medium, Dulbecco’s Phosphate Buffered Saline (DPBS) medium and the bicinchoninic acid (BCA) kit were obtained from Sigma Aldrich Chemie GmbH (Munich, Germany, supplied by Labco LLC. Dubai, UAE). Chloroform was obtained from Panreac Quimica S.A. (Spain). Doxorubicin-hydrochloride was obtained from Euroasian Transcontinental (Lower Parel, Mumbai, India). Sephadex G-100, Sephadex G-25, and Sephacryl S200 HR were obtained from Sigma-Aldrich (Munich, Germany, supplied by Labco LLC. Dubai, UAE). Trastuzumab (Herceptin) was obtained from Hoffmann-La Roche Limited (Basel, Switzerland, supplied by Aster pharmacy, Sharjah, UAE). 2,4,6 trichloro-1,3,5 triazine (cyanuric chloride) was obtained from Sigma-Aldrich (St. Louis, MO, US, supplied by LABCO LLC. Dubai, UAE). SKBR3 (HER2 + cells) and MDA-MB-231 (HER2- cells) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).