Mouse anti human percpcy5.5 cd66b

Four healthy volunteer females 30-40 years old were recruited and informed consent obtained (Stanford University IRB-37618). Negative selection isolation and activation of neutrophils FACs and Flow cytometric analysis Neutrophils were isolated using a negative selection method that allowed for seamless dovetailing with the ATAC-seq method (Fig. 1a, Supplemental Fig. 1). Neutrophils were isolated using the Stem Cell EasySep Direct Human Neutrophil Isolation Kit as per the manufacturer's protocol and resuspended in PBS with 1% FBS and 2mM EDTA. 8.0 x 10 5 cells per condition were fixed in 1% PFA for 10 min at room temperature and subsequently stained with primary antibodies: mouse anti-human PEcy7-CD16 (BD #557744), mouse anti-human PerCPcy5.5-CD66b (Biolegend # 305107), and mouse anti-human V500-CD45 (BD # 560779) as well as the corresponding IgG controls. Flow cytometric and statistical analysis were performed using FlowJo V. 10.0.8.