Video tracker

The light-aversion assay was performed as previously described with modifications (Matynia et al., 2012 (link), 2015 (link)). Briefly, a two-chamber box with an open, light, and closed, dark section was used to measure time spent in the light compartment. An overhead LED lighting system, with adjustable illumination from 0 to 2000 Lux calibrated with a lightmeter (HHLM-2, Omega Engineering Inc.), a standard LED spectrum and diffusers provided uniform illumination in the open, lit side of the chamber. Behavior was monitored using an infrared light source and video camera with white light filter, and automated tracking and analysis were performed with Video Tracker (Med Associates, St. Albans, VT) and Activity Monitor (Med Associates, St. Albans, VT), respectively. Mice were acclimated to a dimly lit room (less that 10 Lux) for at least 45 min and dark-adapted for 10 min prior to testing. Light aversion was tested at 0 and 1000 Lux: the 0 Lux test was used as baseline to calculate aversion indices (AI).

The light-aversion assay was performed as previously described with modifications.⁵⁷ (link)^,58 (link) A two-chamber box with open, light and closed, dark sections was used to measure time spent in the light compartment. An overhead LED lighting system, with adjustable illumination from 0 to 1000 lux calibrated with a light meter (HHLM-2; Omega Engineering, Norwalk, CT, USA), a standard LED spectrum, and diffusers provided uniform illumination in the open, lit side of the chamber. Behavior was monitored using an infrared light source and video camera with white light filter, and automated tracking and analysis were performed with a video tracker (Med Associates, St. Albans, VT, USA) and an activity monitor (Med Associates), respectively. Mice were acclimated to a dimly lit room (less that 10 lux) for at least 15 minutes and dark adapted prior to testing for at least 10 minutes. Light aversion was tested at 0 and 1000 lux: the 0-lux test was used as baseline to calculate aversion indices. A 1% Atropine Sulfate Ophthalmic Solution (Akorn, Lake Forest, IL, USA) was used as a dilating agent where indicated.