Total protein of exosomes, cells, and cardiac tissues were isolated using the RIPA Lysis Buffer (BosterBio, USA). Western blots were performed as previously described.27 (link) The primary antibodies used in this study were as following: Insulin-like growth factor 1 receptor (IGF-IR) (1:500), CD9 (1:500), CD63 (1: 500), Flotillin (1:1000), Tsg101 (1:1000), Rab11a (1:1000), and β-actin (1:5000) (Santa Cruz, Shanghai, China). The protein band was imaged on a Bio-Rad Chemidoc system (Bio-Rad Laboratories, USA).
Igf ir
Manufactured by Santa Cruz Biotechnology
Sourced in China
IGF-IR is a receptor tyrosine kinase that binds insulin-like growth factor I (IGF-I) and mediates the effects of IGF-I on cell growth, survival, and differentiation. It is an important component of the IGF signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Ripa lysis buffer, by Boster Bio (1 mentions)
Chemidoc system, by Bio-Rad (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Rab11a, by Santa Cruz Biotechnology (1 mentions)
Tsg101, by Santa Cruz Biotechnology (1 mentions)
Phospho p44 42 map kinase phospho erk 1 2, by Cell Signaling Technology (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody, by Novus Biologicals (1 mentions)
Ki 67 antibody, by Agilent Technologies (1 mentions)
β actin, by Merck Group (1 mentions)
Appropriate secondary antibodies, by Bio-Rad (1 mentions)
P44 42 map kinase erk 1 2, by Cell Signaling Technology (1 mentions)
2 protocols using igf ir
1
Exosome Protein Analysis by Western Blot
2
Comprehensive Antibody Analysis in Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Throughout the experiments, the following primary antibodies were used: RasGRP3 and phospho-RasGRP3 (Thr133); p44/42 MAP kinase (ERK 1/2) and phospho-p44/42 MAP kinase (phospho-ERK 1/2); Akt and phospho-Akt (Ser473) antibodies for Western blot experiments were obtained from Cell Signaling Technology (Beverly, MA). Anti-RasGRP3 antibody for immunohystochemistry was purchased from Abcam (Cambridge, UK). Ki67 antibody was obtained from DAKO (Glostrup, Denmark). Actin β, ERα, p-ERα (Ser 118) and HER-2 antibodies were purchased from Sigma-Aldrich (St. Louis, MO). IGF-IR and EGFR antibodies were obtained from Santa Cruz Biotechnology (Dallas, TX). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was obtained from Novus Biologicals (Cambridge, UK). Appropriate secondary antibodies were purchased from Bio-Rad Laboratories (Hercules, CA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!