Most images collected using a Leica SP5 confocal microscope. Antibodies used included mouse NeuN (Millipore), rabbit Sema3a (EMD biosciences), goat ChAT (Millipore), rabbit TrkA (gift of L. Reichardt, UCSF), chick GFP (Aveslabs), mouse Parvalbumin (Sigma), chick Neurofilament (Encor), Rabbit Ankyrin G (Santa Cruz), mouse Err3 (PPMX), chick peripherin (Millipore), rabbit Map2 (Millipore) mouse Isl 1/2 (DSHB), sheep Chx10 (abcam) and rabbit calbindin (swant). Rabbit FoxP1 and guinea pig Scip provided by Dr. Jeremy Dasen. In most cases staining was done overnight at 4°C in 5% serum/0.4% Triton, following heat-mediated antigen retrieval for 2 minutes at 95°C in 0.1 M citrate buffer pH 6.0. For GFP labeling Ag retrieval was 10 minutes at 70°C. For TrkA staining, no retrieval was used, and slides were stained overnight at room temperature in 5% serum/1% triton. In situ hybridization performed using standard protocols. Sema3a probe against the full length rat Sema3a transcript provided by Dr. Alex Kolodkin, Wnt7a probe from A. McMahon. Plp probe from Ian Griffiths (Edinburg.)
Chick gfp
Manufactured by Aves Labs
The Chick GFP is a laboratory equipment used for the detection and visualization of green fluorescent protein (GFP) expression in chick embryos. It provides a reliable tool for researchers to study gene expression and developmental processes in avian models.
Automatically generated - may contain errors
Lab products found in correlation
Chick peripherin, by Merck Group (2 mentions)
Rabbit calbindin, by Abcam (2 mentions)
Goat chat, by Merck Group (2 mentions)
Mouse parvalbumin, by Merck Group (2 mentions)
Rabbit ankyrin g, by Santa Cruz Biotechnology (2 mentions)
Mouse err3, by Merck Group (2 mentions)
Rabbit map2, by Merck Group (2 mentions)
Sheep chx10, by Abcam (2 mentions)
Mouse neun, by Merck Group (2 mentions)
Sp5 confocal microscope, by Leica (2 mentions)
2 protocols using chick gfp
1
Comprehensive Neuroanatomical Characterization
2
Comprehensive Neuroanatomical Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Most images collected using a Leica SP5 confocal microscope. Antibodies used included mouse NeuN (Millipore), rabbit Sema3a (EMD biosciences), goat ChAT (Millipore), rabbit TrkA (gift of L. Reichardt, UCSF), chick GFP (Aveslabs), mouse Parvalbumin (Sigma), chick Neurofilament (Encor), Rabbit Ankyrin G (Santa Cruz), mouse Err3 (PPMX), chick peripherin (Millipore), rabbit Map2 (Millipore) mouse Isl 1/2 (DSHB), sheep Chx10 (abcam) and rabbit calbindin (swant). Rabbit FoxP1 and guinea pig Scip provided by Dr. Jeremy Dasen. In most cases staining was done overnight at 4°C in 5% serum/0.4% Triton, following heat-mediated antigen retrieval for 2 minutes at 95°C in 0.1 M citrate buffer pH 6.0. For GFP labeling Ag retrieval was 10 minutes at 70°C. For TrkA staining, no retrieval was used, and slides were stained overnight at room temperature in 5% serum/1% triton. In situ hybridization performed using standard protocols. Sema3a probe against the full length rat Sema3a transcript provided by Dr. Alex Kolodkin, Wnt7a probe from A. McMahon. Plp probe from Ian Griffiths (Edinburg.)
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