Nk 1.1 alexafluor 647

Manufactured by BioLegend

NK-1.1 AlexaFluor 647 is a fluorochrome-conjugated antibody that binds to the NK-1.1 antigen expressed on natural killer cells and a subset of T cells in mice. It can be used for the identification and enumeration of these cell populations by flow cytometry.

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Lab products found in correlation

Cd206 alexa fluor 647, by BioLegend (1 mentions) Cd19 fitc, by BioLegend (1 mentions) F4 80 alexa fluor 488, by BioLegend (1 mentions) Be0089, by BioXCell (1 mentions) Cd4 alexa fluor 647, by BioLegend (1 mentions) Be0146, by BioXCell (1 mentions)

2 protocols using nk 1.1 alexafluor 647

Apoptosis and Cell Cycle Regulation

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NK-1.1 AlexaFluor 647 BioLegend 108720 1:50 (IF); Cleaved Caspase-3 Cell Signaling Technology 9661 1:1000 (IF); Cleaved Caspase-3 Cell Signaling Technology 9664 1:1000 (WB); p21 Abcam 109520 1:1000 (WB); ALDH1A3 Novus Biologicals NBP1-91657 1:1000 (WB) HSP90 Cell Signaling Technology 4877S 1:1000 (WB). The respective RRID of each reagent is listed in the key resources table.

Colucci M., Zumerle S., Bressan S., Gianfanti F., Troiani M., Valdata A., D’Ambrosio M., Pasquini E., Varesi A., Cogo F., Mosole S., Dongilli C., Desbats M.A., Contu L., Revankdar A., Chen J., Kalathur M., Perciato M.L., Basilotta R., Endre L., Schauer S., Othman A., Guccini I., Saponaro M., Maraccani L., Bancaro N., Lai P., Liu L., Pernigoni N., Mele F., Merler S., Trotman L.C., Guarda G., Calì B., Montopoli M, & Alimonti A. (2024). Retinoic acid receptor activation reprograms senescence response and enhances anti-tumor activity of natural killer cells. Cancer Cell, 42(4), 646-661.e9.

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Characterization of Immune Cell Populations

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Therapeutic anti-PD-1 (BE0146) and isotype control antibody (BE0089) were produced by BioXcell. Antibodies used for flow cytometry were purchased from eBioscience (Anti-mouse CD3ε FITC, CD11b eFluor 660) and Biolegend (Anti-mouse CD8a Alexa Fluor® 647, NK 1.1 Alexa Fluor® 647, Ly-6G/Ly-6C (Gr-1) Alexa Fluor® 488, CD19 FITC, CD45R/B220 Alexa Fluor® 647, F4/80 Alexa Fluor® 488, CD206 Alexa Fluor® 647, FOXP3 Alexa Fluor® 488, CD4 Alexa Fluor® 647, CD11c Alexa Fluor® 488, I-A/I-E Alexa Fluor® 647, CD16/32).

Hu H.J., Liang X., Li H.L., Wang H.Y., Gu J.F., Sun L.Y., Xiao J., Hu J.Q., Ni A.M, & Liu X.Y. (2021). Enhanced anti-melanoma efficacy through a combination of the armed oncolytic adenovirus ZD55-IL-24 and immune checkpoint blockade in B16-bearing immunocompetent mouse model. Cancer Immunology, Immunotherapy, 70(12), 3541-3555.

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