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2 protocols using anti pms2 rabbit mab clone epr3947

1

Antibody Selection for Proximity Assays

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Example 1

Example 1 describes non-limiting examples of antibodies used in assays of the present invention.

Reagent anti-HA antibody: Mouse monoclonal Anti-HA-Biotin antibody (clone HA-7) from Sigma-Aldrich (B9183-100UG)

Primary Antibodies used in the proximity assay: anti-E-cadherin rabbit monoclonal antibody, clone EP700Y (Ventana 760-4440), anti-E-cadherin mouse monoclonal antibody clone 36 (Ventana 790-4497), anti-beta-catenin mouse monoclonal antibody clone 14 (Ventana 760-4242), anti-p120 catenin mouse monoclonal antibody clone 98 (Ventana 790-4517), anti-HER-2/neu rabbit monoclonal antibody clone 4B5 (Ventana 790-2991), anti-PR rabbit monoclonal antibody clone 1E2 (Ventana 790-2223), anti-CD21 rabbit monoclonal antibody clone EP3093 (Ventana 760-4438), anti-CD21 mouse monoclonal antibody clone 2G9 (Ventana 760-4245), anti-PMS2 rabbit mAb clone EPR3947 (Ventana 760-4531), anti-MLH1 mouse mAb clone M1 (Ventana 790-4535), anti-CD19 mouse monoclonal antibody clone LE-CD19 (GeneTex, Inc. Irvine, Calif.), anti-phospho-Tyrosine mouse mAb clone P-Tyr-100 and anti-ubiquitin mouse mAb clone P4D1 (Cell Signaling Technology, Inc., Danvers, Mass.).

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2

Antibodies for Cellular Assays

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Example 1

Example 1 describes non-limiting examples of antibodies used in assays of the present invention.

Reagent anti-HA antibody: Mouse monoclonal Anti-HA-Biotin antibody (clone HA-7) from Sigma-Aldrich (B9183-100UG)

Primary Antibodies used in the proximity assay: anti-E-cadherin rabbit monoclonal antibody, clone EP700Y (Ventana 760-4440), anti-E-cadherin mouse monoclonal antibody clone 36 (Ventana 790-4497), anti-beta-catenin mouse monoclonal antibody clone 14 (Ventana 760-4242), anti-p120 catenin mouse monoclonal antibody clone 98 (Ventana 790-4517), anti-HER-2/neu rabbit monoclonal antibody clone 4B5 (Ventana 790-2991), anti-PR rabbit monoclonal antibody clone 1E2 (Ventana 790-2223), anti-CD21 rabbit monoclonal antibody clone EP3093 (Ventana 760-4438), anti-CD21 mouse monoclonal antibody clone 2G9 (Ventana 760-4245), anti-PMS2 rabbit mAb clone EPR3947 (Ventana 760-4531), anti-MLH1 mouse mAb clone M1 (Ventana 790-4535), anti-CD19 mouse monoclonal antibody clone LE-CD19 (GeneTex, Inc. Irvine, Calif.), anti-phospho-Tyrosine mouse mAb clone P-Tyr-100 and anti-ubiquitin mouse mAb clone P4D1 (Cell Signaling Technology, Inc., Danvers, Mass.).

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