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Temperature controller

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The Temperature Controller is a laboratory device designed to precisely regulate and monitor the temperature of a sample or experiment. It features programmable temperature settings, accurate temperature sensing, and responsive control mechanisms to maintain the desired temperature conditions.

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Lab products found in correlation

Vibratome, by Leica (1 mentions)

Spelling variants of this product

TC-324B temperature controller (4 citations) TC-324C temperature controller (2 citations) TC-344D temperature controller (2 citations) CL-100 temperature controller (2 citations) Automatic temperature controller (2 citations)

4 protocols using temperature controller

1

Cardiac action potential recording protocol

Cited 1 time
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APs were recorded under current-clamp mode in normal Tyrode’s solution contained (in mM): NaCl 140, KCl 5.4, CaCl2 1.8, MgCl2 1, glucose 10, HEPES 10, adjusted to pH 7.4 with NaOH. Pipette solution contained (in mM): KCl 130, NaCl 5, MgCl2 1, MgATP 3, EGTA 10, and HEPES 10, adjusted to pH 7.2 with KOH. The parameters of APs include APD at 30%, 50% and 90% of repolarization (APD30, APD50, and APD90), APA, MDP, and beating frequency were analyzed31 (link), 38 (link). Cells were maintained at 35 °C by a temperature controller (Warner Instruments, Hamden, USA) during the recording of APs. The APDs were corrected by the beating frequency (APDc) with Fridericia’s formula (APDc = APD/interspike interval1/3)39 (link).
Koh C.H., Wu J., Chung Y.Y., Liu Z., Zhang R.R., Chong K., Korzh V., Ting S., Oh S., Shim W., Tian H.Y, & Wei H. (2017). Identification of Na+/K+-ATPase inhibition-independent proarrhythmic ionic mechanisms of cardiac glycosides. Scientific Reports, 7, 2465.
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2

Controlled Cooling Stimuli for Neuronal Classification

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For the determination of the neuronal classes based on their cooling threshold6 (link) and also to provide steady state temperature stimuli in the current study, a more controlled type of cooling stimulus was applied to the ocular surface via fluids that flowed into the eye cup (bath). The fluids were drawn out from a reservoir (50-mL beaker) via polyethylene tubing by the use of a peristaltic pump at a rate of 1.3 mL/min and passed through a thermoelectric, Peltier-based device, and pumped into the eye cup (bath). The temperature of the fluids in contact with the ocular surface was regulated by the Peltier-based device (Temperature Controller; Warner Instruments, Hamden, CT, USA), which was placed approximately 1 cm proximal to the eye cup. The cooling stimulus was a 12°C drop from a 31°C adapting temperature of the bath down to 19°C and back to 31°C that took approximately 51 seconds. The rate of cooling was, on average, 0.20°C/s (range, 0.17–0.24) for a 12°C change. The ATs in mM were composed o: NaCl 106.5, NaHCO3 26.1, KCl 18.7, MgCl2 1.0, NaH2PO4 0.5, CaCl2 1.1, HEPES 10, pH 7.45.18 (link) The osmolarities of the solutions were measured with an osmometer (μ OSMETT; Precision System Inc., Natick, MA, USA).
Hirata H., Dallacasagrande V., Mizerska K., Ivakhnitskaia E, & Rosenblatt M.I. (2018). Ambient Air Currents Activate Corneal Nerves During Ocular Desiccation in Rats: Simultaneous Recordings of Neural Activity and Corneal Temperature. Investigative Ophthalmology & Visual Science, 59(10), 4031-4043.
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3

Characterization of Cardiac Action Potentials

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The APs were recorded under current-clamp mode in normal Tyrode’s solution contained (in mM): NaCl 140, KCl 5.4, CaCl2 1.8, MgCl2 1, glucose 10, and HEPES 10, adjusted to pH 7.4 with NaOH. Pipette solution contained (in mM): KCl 130, NaCl 5, MgCl2 1, MgATP 3, EGTA 10, and HEPES 10, adjusted to pH 7.2 with KOH. The parameters of APs include AP durations (APD) at 30%, 50% and 90% of repolarization (APD30, APD50, and APD90), AP amplitude (APA), maximal diastolic potential (MDP), maximal upstroke velocity (dV/dtMax) and beating frequency (BF) were analyzed [18 (link),19 (link)]. The APDs were corrected by heart rates with Fridericia’s formula (APDc = APD/interspike interval1/3) [24 (link)]. Cells were maintained at 35°C by a temperature controller (Warner Instruments, Hamden, USA) during the recording of action potential.
Wu J., Wang X., Chung Y.Y., Koh C.H., Liu Z., Guo H., Yuan Q., Wang C., Su S, & Wei H. (2017). L-Type Calcium Channel Inhibition Contributes to the Proarrhythmic Effects of Aconitine in Human Cardiomyocytes. PLoS ONE, 12(1), e0168435.
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4

Coronal Cortical Slices Preparation

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12- to 14-month-old (G4C2)66/EGFP mice, (G4C2)2/EGFP and EGFP control littermates were sacrificed and their brains rapidly removed and placed into ice-cold artificial cerebrospinal fluid (ACSF) that contained the following (in mΜ): 126 NaCl, 2.5 KCl, 2.5 CaCl2, 1.2 MgCl2, 25 NaHCO3, 1.2 NaH2PO4 and 11 D-glucose. Coronal cortical slices (300 um) containing the dmPFC (including PL and Cg1) were cut using a vibratome (Leica) and superfused with ice-cold ACSF that was saturated with 95% O2 and 5% CO2. Brain slices were incubated at room temperature (21–23 °C) for at least 1 hour prior to being transferred to a recording chamber continuously perfused with oxygenated ACSF and maintained at 32 °C with a temperature controller (Warner Instruments). All drugs used in this study were delivered to the bath by a gravity-driven perfusion system (Harvard Apparatus).
Leikin S., Parsegian V.A., Yang W.H, & Walrafen G.E. (1997). Raman spectral evidence for hydration forces between collagen triple helices. Proceedings of the National Academy of Sciences of the United States of America, 94(21), 11312-11317.
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