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J 028584 12 0020

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J-028584-12-0020 is a laboratory equipment product. It serves as a core function, but a detailed description while maintaining an unbiased and factual approach is not available.

Automatically generated - may contain errors

Lab products found in correlation

Control mirna mimic, by Thermo Fisher Scientific (2 mentions) Let 7a mirna mimic, by Thermo Fisher Scientific (2 mentions) Let 7a inhibitor, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)

2 protocols using j 028584 12 0020

1

Reverse Transfection and Functional Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
BE(2)C and Kelly cells were reverse transfected using Lipofectamine 2000© (Life Technologies) into 6 well plates using the appropriate siRNA or miRNA mimics (described below). Cells were harvested at time-points described for analysis by western or qPCR. Growth assays were performed similarly, but in 96 well plates followed by time-point specific BrdU growth assay. Global let-7 target analysis: BE(2)C:MYCN-ORF cells co-transfected with control or MYCN-3′UTR-2 siRNA and either control or let-7a miRNA mimic were harvested 48 hours post transfection. Control siRNA (Life Technologies #439846). LIN28B siRNAs: ORF1 (Life Technologies #4392420, ID:s52479), ORF2 (Life Technologies #4392420, ID:s52477), 5′UTR (GE Dharmacon Custom LIN28B-NM_001004317 Duplex siRNA, ON-TARGET Plus, sense: 5′-ACU GGA GAG AGG AGA GAA AUU-3′, antisense: 5′-UUU CUC UCC UCU CUC CAG UUU-3′), 3′UTR-1 (GE Dharmacon #J-028584-12-0020), 3′UTR-2 (GE Dharmacon Custom LIN28B-NM_001004317 Duplex siRNA, ON-TARGET Plus, sense: 5′-CAA CAG UGA UUG UGA GAA UUU-3′, antisense: 5′-AUU CUC ACA AUC ACU GUU GUU-3′). MYCN siRNAs: ORF-1 (Life Technologies #4392420, ID:s9135), ORF-2 (Life Technologies #4392420, ID:s9134). Control miRNA mimic (Life Technologies #4464059). let-7a miRNA mimic (Life Technologies #4464066, ID:MC10050), let-7a inhibitor (Life Technologies #4464084, ID:MH10050).
Powers J.T., Tsanov K.M., Pearson D.S., Roels F., Spina C.S., Ebright R., Seligson M., de Soysa Y., Cahan P., Theiβen J., Tu H.C., Han A., Kurek K.C., LaPier G.S., Osborne J.K., Ross S.J., Cesana M., Collins J.J., Berthold F, & Daley G.Q. (2016). Multiple mechanisms disrupt the let-7 microRNA family in neuroblastoma. Nature, 535(7611), 246-251.
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2

Reverse Transfection and Functional Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
BE(2)C and Kelly cells were reverse transfected using Lipofectamine 2000© (Life Technologies) into 6 well plates using the appropriate siRNA or miRNA mimics (described below). Cells were harvested at time-points described for analysis by western or qPCR. Growth assays were performed similarly, but in 96 well plates followed by time-point specific BrdU growth assay. Global let-7 target analysis: BE(2)C:MYCN-ORF cells co-transfected with control or MYCN-3′UTR-2 siRNA and either control or let-7a miRNA mimic were harvested 48 hours post transfection. Control siRNA (Life Technologies #439846). LIN28B siRNAs: ORF1 (Life Technologies #4392420, ID:s52479), ORF2 (Life Technologies #4392420, ID:s52477), 5′UTR (GE Dharmacon Custom LIN28B-NM_001004317 Duplex siRNA, ON-TARGET Plus, sense: 5′-ACU GGA GAG AGG AGA GAA AUU-3′, antisense: 5′-UUU CUC UCC UCU CUC CAG UUU-3′), 3′UTR-1 (GE Dharmacon #J-028584-12-0020), 3′UTR-2 (GE Dharmacon Custom LIN28B-NM_001004317 Duplex siRNA, ON-TARGET Plus, sense: 5′-CAA CAG UGA UUG UGA GAA UUU-3′, antisense: 5′-AUU CUC ACA AUC ACU GUU GUU-3′). MYCN siRNAs: ORF-1 (Life Technologies #4392420, ID:s9135), ORF-2 (Life Technologies #4392420, ID:s9134). Control miRNA mimic (Life Technologies #4464059). let-7a miRNA mimic (Life Technologies #4464066, ID:MC10050), let-7a inhibitor (Life Technologies #4464084, ID:MH10050).
Powers J.T., Tsanov K.M., Pearson D.S., Roels F., Spina C.S., Ebright R., Seligson M., de Soysa Y., Cahan P., Theiβen J., Tu H.C., Han A., Kurek K.C., LaPier G.S., Osborne J.K., Ross S.J., Cesana M., Collins J.J., Berthold F, & Daley G.Q. (2016). Multiple mechanisms disrupt the let-7 microRNA family in neuroblastoma. Nature, 535(7611), 246-251.
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