Human CRC cell lines (HT29, HCT116, SW480, SW837, SW48, SW620 and RKO) were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA). A normal human colon mucosal epithelial cell line (NCM460) and the 293 T cell line was obtained and preserved in our lab. HT29 and HCT116 cells were cultured in McCoy’s 5A (Gibco, Carlsbad, CA, USA), while SW480, SW620, SW48, SW837 and 293 T cells were cultured in DMEM (Gibco, Carlsbad, CA, USA). NCM460 cells were cultured in M3:10 media (INCELL, San Antonio, TX), and RKO cells were cultured with MEM (Gibco, Carlsbad, CA, USA). All culture media contained 10% fetal bovine serum and 1% penicillin. All these cell lines were maintained in a humidified atmosphere of 5% CO2 at 37 °C.
M3 10 media
Manufactured by INCELL
Sourced in United States
M3:10 media is a cell culture media designed for the growth and maintenance of a variety of cell types. It provides the necessary nutrients and growth factors to support cell proliferation and survival in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Sw620, by Thermo Fisher Scientific (3 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (3 mentions)
Mccoy s 5a, by Thermo Fisher Scientific (3 mentions)
Sw837, by Thermo Fisher Scientific (3 mentions)
Sw480, by Thermo Fisher Scientific (3 mentions)
Ht 29, by Thermo Fisher Scientific (3 mentions)
Hct116, by Thermo Fisher Scientific (3 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Hct116, by American Type Culture Collection (3 mentions)
Sw480, by American Type Culture Collection (3 mentions)
Sw620, by American Type Culture Collection (3 mentions)
Sw837, by American Type Culture Collection (3 mentions)
Ncm460, by INCELL (3 mentions)
3 protocols using m3 10 media
1
Colorectal Cancer Cell Line Culture
2
Culturing Diverse Colorectal Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human CRC cell lines (HT29, HCT116, SW480, SW837, SW48, SW620 and RKO) were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA). A normal human colon mucosal epithelial cell line (NCM460) and the 293T cell line was obtained and preserved in our lab. HT29 and HCT116 cells were cultured in McCoy's 5A (Gibco, Carlsbad, CA, USA), while SW480, SW620, SW48, SW837 and 293T cells were cultured in DMEM (Gibco, Carlsbad, CA, USA). NCM460 cells were cultured in M3:10 media (INCELL, San Antonio, TX), and RKO cells were cultured with MEM (Gibco, Carlsbad, CA, USA).
All culture media contained 10% fetal bovine serum and 1% penicillin. All these cell lines were maintained in a humidi ed atmosphere of 5% CO 2 at 37 °C.
All culture media contained 10% fetal bovine serum and 1% penicillin. All these cell lines were maintained in a humidi ed atmosphere of 5% CO 2 at 37 °C.
3
Culture of Colorectal Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human CRC cell lines (HT29, HCT116, SW480, SW837, SW48, SW620 and RKO) were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA). A normal human colon mucosal epithelial cell line (NCM460) and 293 T cell lines were obtained and preserved in our lab. HT29 and HCT116 cells were cultured in McCoy's 5A (Gibco, Carlsbad, CA, USA), SW480, SW620, SW48, SW837 and 293 T cells were cultured in DMEM (Gibco, Carlsbad, CA, USA), NCM460 cells were cultured in M3:10 media (INCELL, San Antonio, TX), and RKO cells were cultured with MEM (Gibco, Carlsbad, CA, USA), all culture medium containing 10% fetal bovine serum and 1% penicillin. All these cell lines were maintained in a humidified atmosphere of 5% CO2 at 37 °C.
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