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Rnapli ser5p antibody 4h8

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The RNAPlI-Ser5P antibody [4H8] is a laboratory reagent used to detect and study the serine 5 phosphorylated form of the largest subunit of RNA polymerase II. This antibody is designed to recognize the phosphorylated state of this enzyme, which is involved in the regulation of transcription initiation and elongation.

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Lab products found in correlation

Rnapolii ser2p antibody chip grade, by Abcam (2 mentions) Ampure bead, by Beckman Coulter (2 mentions) Nextseq platform, by Illumina (2 mentions)

2 protocols using rnapli ser5p antibody 4h8

1

ChIP-seq Workflow for Transcription Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP was performed as previously described32 using the following antibodies: RNAPolII-Ser2P antibody - ChIP Grade (Abcam ab5095), RNAPlI-Ser5P antibody [4H8] (Abcam ab5408), and anti-HP1γ antibody, clone 42s2 (05–690 from Merck Millipore). Libraries were size selected with AMPure beads (Beckman Coulter) for 200–800 base pair size range and quantified by qPCR using a KAPA Library Quantification Kit. ChIP-seq data were generated using the NextSeq platform from Illumina with 2 × 75 bp Hi Output (all samples pooled, and sequenced on four consecutive runs before merger of FASTQ files).
Yoshimi A., Lin K.T., Wiseman D.H., Rahman M.A., Pastore A., Wang B., Lee S.C., Micol J.B., Zhang X.J., de Botton S., Penard-Lacronique V., Stein E.M., Cho H., Miles R.E., Inoue D., Albrecht T.R., Somervaille T.C., Batta K., Amaral F., Simeoni F., Wilks D.P., Cargo C., Intlekofer A.M., Levine R.L., Dvinge H., Bradley R.K., Wagner E.J., Krainer A.R, & Abdel-Wahab O. (2019). Coordinated Alterations in RNA Splicing and Epigenetic Regulation Drive Leukemogenesis. Nature, 574(7777), 273-277.
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2

ChIP-seq Workflow for Transcription Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP was performed as previously described32 using the following antibodies: RNAPolII-Ser2P antibody - ChIP Grade (Abcam ab5095), RNAPlI-Ser5P antibody [4H8] (Abcam ab5408), and anti-HP1γ antibody, clone 42s2 (05–690 from Merck Millipore). Libraries were size selected with AMPure beads (Beckman Coulter) for 200–800 base pair size range and quantified by qPCR using a KAPA Library Quantification Kit. ChIP-seq data were generated using the NextSeq platform from Illumina with 2 × 75 bp Hi Output (all samples pooled, and sequenced on four consecutive runs before merger of FASTQ files).
Yoshimi A., Lin K.T., Wiseman D.H., Rahman M.A., Pastore A., Wang B., Lee S.C., Micol J.B., Zhang X.J., de Botton S., Penard-Lacronique V., Stein E.M., Cho H., Miles R.E., Inoue D., Albrecht T.R., Somervaille T.C., Batta K., Amaral F., Simeoni F., Wilks D.P., Cargo C., Intlekofer A.M., Levine R.L., Dvinge H., Bradley R.K., Wagner E.J., Krainer A.R, & Abdel-Wahab O. (2019). Coordinated Alterations in RNA Splicing and Epigenetic Regulation Drive Leukemogenesis. Nature, 574(7777), 273-277.
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