Anti nox2

Manufactured by Proteintech

Anti-NOX2 is a laboratory product that detects the NOX2 protein. NOX2 is an enzyme involved in the production of reactive oxygen species. This product can be used to measure the levels of NOX2 in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Anti nox4, by Proteintech (2 mentions) Anti lamin a c, by Cell Signaling Technology (1 mentions) Hrp conjugated affinipure goat anti mouse igg, by Proteintech (1 mentions) Anti sirt1, by Abcam (1 mentions) Anti vwf, by Santa Cruz Biotechnology (1 mentions) Selisistat, by MedChemExpress (1 mentions) Alexa fluor 647 phalloidin, by Thermo Fisher Scientific (1 mentions) Hrp conjugated affinipure goat anti rabbit igg, by Proteintech (1 mentions) Anti lamin b1, by Proteintech (1 mentions) Anti cd31 pecam 1, by Santa Cruz Biotechnology (1 mentions) Resveratrol, by MedChemExpress (1 mentions) Anti progerin, by Santa Cruz Biotechnology (1 mentions) Anti α sma, by Boster Bio (1 mentions) Anti p53, by Abcam (1 mentions) Anti histone h3, by Proteintech (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Carbon tetrachloride, by Merck Group (1 mentions) Anti gapdh, by Proteintech (1 mentions) Anti cd31, by Abcam (1 mentions) Anti cd31, by Proteintech (1 mentions)

Spelling variants of this product

Anti-TM (2 citations)

3 protocols using anti nox2

Detailed Procedures for Cellular Senescence Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols

The reagents included carbon tetrachloride (Sigma‐Aldrich, 56‐23‐5), 30% H₂O₂ (Hydrogen peroxide 30%, Sigma‐Aldrich, 1.07298), selisistat (EX‐527, MedChemExpress, 49843‐98‐3), resveratrol (SRT501, MedChemExpress, 501‐36‐0), DAPI (Sigma‐Aldrich, D9542), Alexa Fluor™ 647 Phalloidin (Thermo, A22287), protease cocktails inhibitor (Beyotime, P1005) and PMSF (Phenylmethanesulphonyl fluoride, Beyotime, ST506).
The primary antibodies included anti‐α‐SMA (Boster, BM0002), anti‐vWF (Santa Cruz, SC‐365712), anti‐CD32b (ZEN‐bioscience, 382560), anti‐CD31 (PECAM‐1, Santa Cruz, sc‐18916), anti‐CD31 (Abcam, ab33858), anti‐NOX2 (Proteintech, 19013‐1‐AP), anti‐NOX4 (Proteintech, 14347‐1‐AP), anti‐Lamin A/C (Cell Signaling Technology, 4777S), anti‐Lamin B1 (Proteintech, 66095‐1‐Ig), anti‐progerin (Santa Cruz, sc‐81611), anti‐p53 (Abcam, ab131442), anti‐p53 (acetyl K381; Abcam, ab61241), anti‐SIRT1 (Abcam, ab110304), anti‐Histone H3 (Proteintech, 17168‐1‐AP) and anti‐GAPDH (Proteintech, 60004‐1). HRP‐conjugated Affinipure Goat Anti‐Mouse IgG (H + L; Proteintech, SA00001‐1), HRP‐conjugated Affinipure Goat Anti‐Rabbit IgG (H + L; Proteintech, SA00001‐2), FITC‐labelled goat anti‐rabbit IgG (H + L; Beyotime, a0562) and Cy3‐labelled goat anti‐mouse IgG (H + L; Beyotime, a0521) were used for secondary antibodies.

Luo X., Bai Y., He S., Sun S., Jiang X., Yang Z., Lu D., Wei P., Liang Y., Peng C., Wang Y., Sheng R., Han S., Li X, & Zhang B. (2021). Sirtuin 1 ameliorates defenestration in hepatic sinusoidal endothelial cells during liver fibrosis via inhibiting stress‐induced premature senescence. Cell Proliferation, 54(3), e12991.

+ Open protocol

+ Expand

Comprehensive Antibody Panel for Vascular Endothelial Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies include anti-KCa3.1 (Abcam, ab229593, and Alomone Labs, #ALM-051), anti-p-eNOS (Cell Signaling Technology, #9571s), t-eNOS (Cell Signaling Technology, #9572s), anti-TM (Proteintech, 14318-1-AP), anti-NOX2 (Proteintech, 19013-1-AP), anti-SOD1 (Proteintech, 10269-1-AP), anti-CD31 (Proteintech, 66065-1-Ig), GPx1 (Cell Signaling Technology, #3206S), anti-4HNE (Abcam, ab46545), anti-β-actin (Santa Cruz, sc-47778), and anti-GAPDH (Santa Cruz, sc-47724).

Li X., Liu Y., Cao A., Li C., Wang L., Wu Q., Li X., Lv X., Zhu J., Chun H., Laba C., Du X., Zhang Y, & Yang H. (2021). Crocin Improves Endothelial Mitochondrial Dysfunction via GPx1/ROS/KCa3.1 Signal Axis in Diabetes. Frontiers in Cell and Developmental Biology, 9, 651434.

+ Open protocol

+ Expand

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole-cell lysates were obtained by resuspending cell pellets in RIPA buffer (50 mmol/L Tris pH 7.4, 150 mmol/L NaCl, 1% Triton X-100) with freshly added protease inhibitor (Roche). Nuclear proteins were extracted essentially as described before. 23 (link) Antibodies were incubated with cell lysates overnight before being absorbed by Protein A/G-plus Agarose beads. Precipitated immune complex was released by boiling with 1X electrophoresis sample buffer. Western blot analyses were performed with anti-MKL1 (Santa Cruz), anti-MOF (Bethyl Laboratories), anti-NOX1, anti-NOX2, anti-NOX4 (Proteintech), and anti-β-actin (Sigma) antibodies.

Yu L., Yang G., Zhang X., Wang P., Weng X., Yang Y., Li Z., Fang M., Xu Y., Sun A, & Ge J. (2018). Megakaryocytic Leukemia 1 Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury. Circulation, 138(24).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!