R26dta

Manufactured by Jackson ImmunoResearch

The R26DTA is a laboratory instrument designed for the detection and quantification of specific analytes in samples. It utilizes a fluorescence-based detection method to provide reliable and accurate results. The core function of the R26DTA is to assist researchers and scientists in their analytical procedures.

Automatically generated - may contain errors

Lab products found in correlation

Rosa26dta, by Jackson ImmunoResearch (1 mentions) Cx3cr1creer eyfp, by Jackson ImmunoResearch (1 mentions) R26 tdtomato, by Jackson ImmunoResearch (1 mentions) R26 confetti, by Jackson ImmunoResearch (1 mentions) C57bl 6 cd45.2, by Jackson ImmunoResearch (1 mentions) Rag2 il2rg, by Taconic Biosciences (1 mentions) B6sjl cd45.1, by Taconic Biosciences (1 mentions)

4 protocols using r26dta

Spatiotemporal Regulation of Prostaglandin Synthesis in Histaminergic Neurons

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

All mouse experiments were conducted under the Institutional Animal Care and Use Committee protocols AABE1556 and AABT7651 at the Columbia University Irving Medical Center, and all mouse studies were approved by the Columbia University Institutional Animal Care and Use Committee. For all experiments, male and female mice were used at 8–12 weeks of age at the beginning of each experiment. Hdc^GFP and Hdc^CreERT2 mice have been described previously.³⁷ (link)^,³⁸ (link)
Hdc^CreERT2 was mated to Rosa26-DTA (007909; Jackson Labs) (R26^DTA) mice for generating Hdc^CreERT2; R26^DTA mice. Hdc^Cre mice were purchased from Jackson Labs (021198).⁶⁵ (link)
Ptgs2^fl/fl mice were gifted by Harvey Herschman.⁴⁵ (link)
Hdc^Cre was mated to Ptgs2^fl/fl to generate Hdc^Cre; Ptgs2^fl/fl mice. All mice were maintained in a C57BL/6J background.

Jiang Z., Waterbury Q.T., Malagola E., Fu N., Kim W., Ochiai Y., Wu F., Guha C., Shawber C.J., Yan K.S, & Wang T.C. (2023). Microbial-Dependent Recruitment of Immature Myeloid Cells Promotes Intestinal Regeneration. Cellular and Molecular Gastroenterology and Hepatology, 17(3), 321-346.

+ Open protocol

+ Expand

Transgenic Mouse Colonies for Neuroscience

Check if the same lab product or an alternative is used in the 5 most similar protocols

All mice were bred and maintained under specific pathogen-free conditions at Karolinska Institutet, in accordance with national animal care guidelines. All animal experiments were approved by the appropriate ethical review board (Stockholms djurförsöksetiska nämnd). Cx3cr1^CreER-EYFP, R26^DTR, R26^DTA, CD45.1 were originally obtained from The Jackson Laboratory. Cx3cr1^GFP/+Ccr2^RFP/+, and Ccr2^{–/– (RFP/RFP)} mice were a gift from Klas Blomgren at Karolinska Institutet. Ifnar1^–/– were originally obtained from Ulrich Kalinke. Experiments were started when mice were 6–12 weeks.

Lund H., Pieber M., Parsa R., Han J., Grommisch D., Ewing E., Kular L., Needhamsen M., Espinosa A., Nilsson E., Överby A.K., Butovsky O., Jagodic M., Zhang X.M, & Harris R.A. (2018). Competitive repopulation of an empty microglial niche yields functionally distinct subsets of microglia-like cells. Nature Communications, 9, 4845.

+ Open protocol

+ Expand

Ascl3-EGFP-Cre Lineage Tracing

Check if the same lab product or an alternative is used in the 5 most similar protocols

All mice were maintained on a C57BL/6 J background. Generation of the Ascl3^EGFP-Cre/+ mice was done by replacing exon 2, including the entire coding region, with a fusion cassette encoding nuclear EGFP and Cre recombinase29 (link). The Ascl3^EGFP-Cre/+ mice were crossed with the R26^tdTomato (129S-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J) reporter strain, R26^Confetti (Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J) reporter strain and the inducible cell ablation R26^DTA (Gt(ROSA)26Sor^tm1(DTA)Jpmb/J) strain (obtained from Jackson Laboratory). Genotyping was performed with following primers: Ascl3: forward 5′-CCACCCCAGTGCCTCTACACAAAT-3′, reverse 5′-GTCGCTGGAGAAGGGCAGCAGA-3′, and Cre reverse 5′-GGTGTACGGTCAGTAAATTGGAC-3. Genotyping of R26^tdTomato, R26^Confetti and R26^DTA mice was done with primers as recommended by supplier (Jackson Laboratory). Mice were maintained on a 12-hour light/dark cycle in a one-way, pathogen-free facility at the University of Rochester Medical Center. Food and water were provided ad libitum. All procedures were approved and conducted in accordance with the University of Rochester IACUC.

Weng P.L., Vinjamuri M, & Ovitt C.E. (2016). Ascl3 transcription factor marks a distinct progenitor lineage for non-neuronal support cells in the olfactory epithelium. Scientific Reports, 6, 38199.

+ Open protocol

+ Expand

Genetic Mouse Model Infections

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

All mice used in this study were bred and maintained at Memorial Sloan Kettering Cancer Center (MSKCC) in accordance with all guidelines of the Institutional Animal Care and Use Committee. This study used the following mouse strains, all on the C57BL/6 genetic background: C57BL/6 (CD45.2; The Jackson Laboratory), B6.SJL (CD45.1; Taconic), Ifnar1^−/− (Müller et al., 1994 (link)), Stat1^−/− (Meraz et al., 1996 (link)), Klra8^−/− (Ly49H deficient; Fodil-Cornu et al., 2008 (link)), Nkp46^iCre (referred to as NKp46^Cre; Narni-Mancinelli et al., 2011 (link)), B2m^−/− (Taconic), Rag2^−/−Il2rg^−/− (Taconic), Ncr1^gfp/gfp (Gazit et al., 2006 (link)), Prf1^−/− (The Jackson Laboratory), and R26^DTA (The Jackson Laboratory). NKp46^Crex R26^DTA mice were generated at MSKCC. Adoptive transfer studies and the generation of mixed bone marrow chimeric mice were performed as previously described (Sun et al., 2009 (link)). Bone marrow chimeric mice were infected by i.p. injections of 7.5 × 10³ PFU of salivary gland–derived Smith strain MCMV. Mice used in adoptive transfer studies were infected with 7.5 × 10² PFU of MCMV. Newborn Ly49H-deficient mice were infected with 2 × 10³ PFU of MCMV. LCMV infection was performed as described previously (Sun et al., 2004 (link)). In vivo blockade of NKG2D signaling was accomplished by i.p. injection of anti-NKG2D (clone CX5; 200 µg/mouse) on day 0 of LCMV infection.

Madera S., Rapp M., Firth M.A., Beilke J.N., Lanier L.L, & Sun J.C. (2016). Type I IFN promotes NK cell expansion during viral infection by protecting NK cells against fratricide. The Journal of Experimental Medicine, 213(2), 225-233.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!