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Rhodobacter sphaeroides

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Rhodobacter sphaeroides is a species of purple non-sulfur bacteria. It is a Gram-negative, photosynthetic bacterium that can grow under both aerobic and anaerobic conditions. Rhodobacter sphaeroides is commonly used in research laboratories for studying photosynthesis, bacterial metabolism, and genetic engineering.

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Lab products found in correlation

Bacillus cereus, by American Type Culture Collection (3 mentions) Escherichia coli, by American Type Culture Collection (3 mentions) Bifidobacterium adolescentis, by American Type Culture Collection (2 mentions) Staphylococcus epidermidis, by American Type Culture Collection (2 mentions) Lactobacillus gasseri, by American Type Culture Collection (2 mentions) Streptococcus mutans, by American Type Culture Collection (2 mentions) Deinococcus radiodurans, by American Type Culture Collection (2 mentions) Enterococcus faecalis, by American Type Culture Collection (2 mentions) Staphylococcus aureus, by American Type Culture Collection (2 mentions) Acinetobacter baumannii, by American Type Culture Collection (1 mentions) Helicobacter pylori, by American Type Culture Collection (1 mentions) Cutibacterium acnes, by American Type Culture Collection (1 mentions) Streptococcus agalactiae, by American Type Culture Collection (1 mentions) Bacteroides vulgatus, by American Type Culture Collection (1 mentions) Porphyromonas gingivalis, by American Type Culture Collection (1 mentions) Neisseria meningitidis, by American Type Culture Collection (1 mentions) Pseudomonas aeruginosa, by American Type Culture Collection (1 mentions) Neisseria gonorrhoeae, by American Type Culture Collection (1 mentions) Bacillus subtillis, by American Type Culture Collection (1 mentions)

3 protocols using rhodobacter sphaeroides

1

Characterization of Bacterial DNA Mixture

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A mixture of bacterial DNA (10 Strain Even Mix Genomic Material, MSA-1000) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), comprising genomic DNA prepared from the following ten bacterial strains: Bacillus cereus (ATCC 10987), Bifidobacterium adolescentis (ATCC 15703), Clostridium beijerinckii (ATCC 35702), Deinococcus radiodurans (ATCC BAA­816), Enterococcus faecalis (ATCC 47077), Escherichia coli (ATCC 700926), Lactobacillus gasseri (ATCC 33323), Rhodobacter sphaeroides (ATCC 17029), Staphylococcus epidermidis (ATCC 12228), and Streptococcus mutans (ATCC 700610).
Matsuo Y., Komiya S., Yasumizu Y., Yasuoka Y., Mizushima K., Takagi T., Kryukov K., Fukuda A., Morimoto Y., Naito Y., Okada H., Bono H., Nakagawa S, & Hirota K. (2021). Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution. BMC Microbiology, 21, 35.
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2

Multi-Strain Bacterial Reference Sample

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MSA-2002 was purchased from ATCC, Manassas, VA. The sample contains a mixture of 20 different bacterial strains distributed equally (5% ea.): Acinetobacter baumannii (ATCC 17978), Actinomyces odontolyticus (ATCC 17982), Bacillus cereus (ATCC 10987), Bacteroides vulgatus (ATCC 8482), Bifidobacterium adolescentis (ATCC 15703), Clostridium beijerinckii (ATCC 35702), Cutibacterium acnes (ATCC 11828), Deinococcus radiodurans (ATCC BAA-816), Enterococcus faecalis (ATCC 47077), Escherichia coli (ATCC 700926), Helicobacter pylori (ATCC 700392), Lactobacillus gasseri (ATCC 33323), Neisseria meningitidis (ATCC BAA-335), Porphyromonas gingivalis (ATCC 33277), Pseudomonas aeruginosa (ATCC 9027), Rhodobacter sphaeroides (ATCC 17029), Staphylococcus aureus (ATCC BAA-1556), Staphylococcus epidermidis (ATCC 12228), Streptococcus agalactiae (ATCC BAA-611), and Streptococcus mutans (ATCC 700610).
Deshpande S.V., Reed T.M., Sullivan R.F., Kerkhof L.J., Beigel K.M, & Wade M.M. (2019). Offline Next Generation Metagenomics Sequence Analysis Using MinION Detection Software (MINDS). Genes, 10(8), 578.
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3

Evaluating DNA Signature Specificity Across Species

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Genomic DNA from nonhuman organisms were tested in species specificity studies: two Old World primates (female baboon, Zyagen Laboratories, San Diego, CA; male rhesus monkey, BioChain 1 , Eureka, CA), nine non-primate mammals (male cat, male dog, mixed male and female ferret, female horse (Zyagen Laboratories, San Diego, CA)), and male cow, mixed male and female hamster, male mouse, male pig, male rat (BioChain 1 , Eureka, CA), one avian species (male domesticated chicken; Zyagen Laboratories, San Diego, CA), two fungal samples (Candida albicans, BioChain 1 , Eureka, CA; Saccharomyces cerevisiae, White Labs, San Diego, CA), and a pooled bacterial sample of six microorganisms that was treated as a single sample (Rhodobacter sphaeroides, Escherichia coli, Bacillus cereus; ATCC, Manassas, VA), Staphylococcus aureus, Neisseria gonorrhoeae, Bacillus subtillis (Cambridge University Hospital, Cambridge, United Kingdom). Baboon and monkey (1 ng) and all other nonhuman samples (10 ng) were amplified with DPMB and processed according to the ForenSeq TM DNA Signature Prep Guide (Illumina part #15049528) [77] .
Jäger A.C., Alvarez M.L., Davis C.P., Guzmán E., Han Y., Way L., Walichiewicz P., Silva D., Pham N., Caves G., Bruand J., Schlesinger F., Pond S.J.K., Varlaro J., Stephens K.M, & Holt C.L. (2017). Developmental validation of the MiSeq FGx Forensic Genomics System for Targeted Next Generation Sequencing in Forensic DNA Casework and Database Laboratories. Forensic science international. Genetics, 28.
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