Primary antibody dilution buffer p0023a

Manufactured by Beyotime

Primary antibody dilution buffer (P0023A) is a laboratory reagent used to dilute primary antibodies prior to their application in various immunoassays. It is designed to maintain the stability and specificity of the primary antibodies during the dilution process.

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Lab products found in correlation

Phospho nf κb p65 ser536, by Cell Signaling Technology (1 mentions) Phospho nf κb p65 ser468, by Cell Signaling Technology (1 mentions) Anti mouse igg 7076p2, by Cell Signaling Technology (1 mentions) Gtx55960, by GeneTex (1 mentions) β actin, by Proteintech (1 mentions)

Spelling variants of this product

Primary antibody dilution buffer (77 citations) Antibody dilution buffer (13 citations) Dilution buffer (9 citations)

2 protocols using primary antibody dilution buffer p0023a

Western Blot Antibody Panel for Cell Signaling

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Primary antibodies for detecting DHFR (15194-1-AP), β actin (60008-1-Ig), CDK2 (10122-1-AP), CDK4 (11026-1-AP), CDK6 (14052-1-AP), Cyclin D1 (60186-1-Ig), Cyclin E (11554-1-AP), and p21^waf/cip1 (10355-1-AP) were purchased from Proteintech (Chicago, IL). Antibodies for detection of p27^Kip1 (3686), p53 (2527), NF-κB/p65 (6956), phospho-NF-κB/p65 (Ser468) (3039), phospho-NF-κB/p65 (Ser536) (3033), Akt (4691), phospho-Akt (4060), p38 MAPK (9212), phospho-p38 MAPK (4511), p44/42 MAPK (Erk1/2) (4695), phospho-p44/42 MAPK (Erk1/2) (4370), SAPK/JNK (9258), and phospho-SAPK/JNK (4668) were purchased from Cell Signaling Technology (Danvers, MA). Horseradish peroxidase (HRP)-linked secondary antibodies, anti-rabbit IgG (7074P2) and anti-mouse IgG (7076P2), were obtained from Cell Signaling Technology. RIPA lysis buffer (P0013B), phenylmethanesulfonyl fluoride (PMSF) (ST506), and primary antibody dilution buffer (P0023A) were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Protease inhibitor cocktail (BS387) was purchased from Sangon Biotech (Shanghai, China). 5-Methyltetrahydrofolic acid disodium salt (M0132) were obtained from Sigma-Aldrich (St. Louis, MO).

Fei Z., Gao Y., Qiu M., Qi X., Dai Y., Wang S., Quan Z., Liu Y, & Ou J. (2016). Down-regulation of dihydrofolate reductase inhibits the growth of endothelial EA.hy926 cell through induction of G1 cell cycle arrest via up-regulating p53 and p21waf1/cip1 expression. Journal of Clinical Biochemistry and Nutrition, 58(2), 105-113.

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Western Blot Analysis of NEIL3

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Cell lysis, the measurement of lysate protein concentration, and Western blotting were conducted as previously described [21 (link)]. Primary Antibody Dilution Buffer (P0023A, Beyotime) was used to dilute primary antibodies, including NEIL3 (GTX55960, GeneTex, 1:1000), β-actin (60008-1-Ig, Proteintech, 1:5000), and GAPDH (D16H11, CST, 1:1000).

Liao W., Huang S., Li L., Wang J., Li J., Chen Y., Chen L., Lian Y, & Huang Y. (2022). Pan-Cancer Landscape of NEIL3 in Tumor Microenvironment: A Promising Predictor for Chemotherapy and Immunotherapy. Cancers, 15(1), 109.

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