For immunochemistry analysis, hepatic tissue section slides were incubated with anti-BAX rabbit polyclonal antibody (Servicebio biotechnology corp., Wuhan, China) and anti-Bcl-xL rabbit monoclonal antibody (Abcam, Cambridge, UK). After incubation with the primary antibody overnight at 4 °C, the slides were washed with PBS (pH 7.4) 3 times and incubated with goat anti-rabbit secondary antibody for 50 min under room temperature. Then, the slides were stained with diaminobenzidine tetrahydrochloride and counterstained with hematoxylin for 3 min. After dehydration and mounting, the slides were visualized under a Leica microscope (Leica DMi8; Leica Corp., Weztlar, Germany). The image pro plus 6.0 software (Media Cybernetics, Rockville, MD, USA) was used to analyze the Integrated Optical Density (IOD) and area of the positive cells to determine the relative protein expression ratio (IOD/Area).
Anti bax rabbit polyclonal antibody
Manufactured by Wuhan Servicebio Technology
Sourced in China
The Anti-BAX rabbit polyclonal antibody is a laboratory reagent used for the detection and analysis of the BAX protein in various biological samples. It is produced by immunizing rabbits with a specific antigen and purifying the resulting antibodies.
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2 protocols using anti bax rabbit polyclonal antibody
1
Immunohistochemical Analysis of Hepatic Apoptosis
2
Immunohistochemical Evaluation of Oxidative Stress, Inflammation, and Apoptosis
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Immunohistochemical staining was performed to evaluate the expression of oxidative stress (heme oxygenase-1 (HO-1)), inflammatory responses (tumor necrosis factor-alpha (TNF-α)) and apoptotic proteins (Bax). Paraffin-embedded sections were dewaxed and hydrated. After antigen repair in the citric acid solution, the sections were incubated in 3% hydrogen peroxide for 25 minutes to neutralize the endogenous peroxidase activity. Then sections were treated with goat serum containing 3% bovine serum albumin for 30 minutes and further incubated with primary antibodies (anti-HO-1 rabbit polyclonal antibody, dilution of 1:100, Cat# GB11104; anti-TNF-α rabbit polyclonal antibody, dilution of 1:500, Cat# GB11188; anti-Bax rabbit polyclonal antibody, dilution of 1:100, Cat# GB11007-1; all provide by Servicebio, Wuhan, China) at 4°C overnight. This was followed by incubation with a specific secondary antibody (goat anti-rabbit, Cat# GB23303, dilution of 1:200, Servicebio) at room temperature for 50 minutes. Finally, the sections were visualized using diaminobenzidine substrate liquid and the chromogenic reaction observed under a microscope. Images were recorded at an original magnification of 400× using an Olympus BX53 light microscope.
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