Quadrupole mass spectrometer model 5975c

The aqueous ammonia Q. suber bark extract was studied by gas chromatography–mass spectrometry (GC-MS) at the Research Support Services (STI) at Universidad de Alicante (Alicante, Spain), using a gas chromatograph model 7890A coupled to a quadrupole mass spectrometer model 5975C (both from Agilent Technologies, Santa Clara, CA, USA). Chromatographic conditions were as follows: 3 injections/vial, injection volume = 1 µL; injector temperature = 280 °C, in splitless mode; initial oven temperature = 60 °C, after 2 min, followed by an increase of 10 °C/min up to a final temperature of 300 °C, after 15 min. The chromatographic column used for the separation of the compounds was an Agilent Technologies HP-5MS UI of 30 m in length, 0.250 mm in diameter, and with 0.25 µm film. The conditions of the mass spectrometer were as follows: temperature of the electron impact source of the mass spectrometer = 230 °C and of the quadrupole = 150 °C; ionization energy = 70 eV. Test mixture 2 for apolar capillary columns according to Grob (Supelco 86501) and PFTBA tuning standards were used for equipment calibration. The identification of the components was based on a comparison of their mass spectra and retention time with those of the authentic compounds and by computer matching with the database of the National Institute of Standards and Technology (NIST11) and Adams [83 ].

The aqueous ammonia extract of oak bark was analyzed by gas chromatography-mass spectrometry (GC–MS) at the Research Support Services (STI) at the University of Alicante (Alicante, Spain), using a gas chromatograph model 7890A coupled to a quadrupole mass spectrometer model 5975C (both from Agilent Technologies, Santa Clara, CA, USA). The operating conditions were: injector temperature = 280 °C, splitless mode; injection volume = 1 µL; initial temperature = 60 °C, 2 min, followed by a ramp of 10 °C·min⁻¹ to a final temperature of 300 °C, 15 min. The chromatographic column used for the separation of the compounds was an Agilent Technologies HP-5MS UI of 30 m in length, 0.250 mm diameter, and 0.25 µm film. The mass spectrometer conditions were: mass spectrometer electron impact source temperature = 230 °C and quadrupole temperature = 150 °C; ionization energy = 70 eV. Test mixture 2 for apolar capillary columns according to Grob (Supelco 86501) and PFTBA tuning standards were used for calibration, purchased from Sigma Aldrich Química S.A. (Madrid, Spain). Comparison of mass spectra and retention times with those of reference compounds and computer matching with the databases of the National Institute of Standards and Techniques (NIST11) and the monograph by Adams [61 ] were used for compound identification.