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2 protocols using 3 4 ethylenedioxythiophene edot

1

Synthesis and Characterization of PEDOT-Based Polymer

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3,4-Ethylenedioxythiophene (EDOT) and (2,3-dihydrothieno [3,4-b][1,4]dioxin-2-yl)methanol were obtained from AK scientific, oligoethylene glycol methyl ether methacrylate (OEGMA), 2-bromopropionyl bromide, copper bromide, 2,2′-bipyridine and lithium perchlorate were purchased from Sigma-Aldrich (Auckland, New Zealand). Dichloromethane, acetonitrile, sodium bicarbonate, magnesium sulphate, ethanol and methanol were provided by ECP Ltd. (Auckland, New Zealand). Triethylamine (TEA) was purchased from BDH (Poole, UK). Bovine serum albumin (BSA) was acquired from pH Scientific (Auckland, New Zealand). PierceTM Bicinchoninic acid (BCA) Protein Assay Kit was purchased from ThermoFisher Scientific (Auckland, New Zealand). Iron (III) chloride was obtained from ECP Ltd. and all the deionised water was obtained from MilliQ (18.2 MΩ cm) system (Arium Pro Ultrapure Water Systems, Sartorius, Germany).
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2

Biomaterial Synthesis and Characterization

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Phosphate-Buffered Saline (PBS), Fetal Bovine Serum (FBS), Dulbecco’s Modified Eagle Medium (DMEM), LIVE/DEAD® Viability/Cytotoxicity Kit, Alexa Fluor 488 actin conjugate (F-actin staining), and antibodies (sarcomeric α-actin, connexin-43 (Cx-43)) were purchased from Life Technologies. NBR with 44% acetonitrile (ACN) was kindly provided by Cédric Plesse from LPPI, Institut des Materiaux who received it from Lanxess (Mw=230 000 gmol−1, Per-bunan 4456F). Chloroform, methanol, benzoyl peroxide (BPO), poly(ethylene glycol) dimethacrylate (PEGDM, Mn=550 gmol−1), 2,2-dimethoxy-2-phenylacetophenone (Irgacure 651), acetone, poly(ethylene oxide) (PEO, 100 000 D) and phosphate buffer saline solution were purchased from Sigma Aldrich. Anhydrous iron III chloride was purchased from Acros, 3,4-ethylenedioxythiophene (EDOT) was purchased from AK Scientific. EDOT was doubly distilled under reduced pressure before use. All materials were used as received unless otherwise stated.
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