Cells were seeded onto a 60-mm dish at 1×106 cells/dish and treated with various reagents for 48 h. Then, the cells were fixed for 1 h at 4°C with 2.5% glutaraldehyde (TAAB Laboratories Equipment, Ltd.) in 0.1 M phosphate buffer (pH 7.3) (prepared with monobasic sodium phosphate and dibasic sodium phosphate; Wako Pure Chemical Industries), and fixed subsequently for 1 h at RT in 1% osmium tetroxide (Nisshin EM Co., Ltd.), dehydrated in graded ethanol (30-100%), and embedded in Quetol 812 epoxy resin (Nisshin EM Co., Ltd.) at 60°C for 2-4 days. ultrathin sections (60 nm) were obtained using an Ultracut J micro-tome (Reichert Jung), and the sections were stained with 4% lead nitrate (RT, 5 min) and saturated uranium acetate (RT, 10 min) and imaged using a transmission electron micro-scope JEM-1200EX II (JEOL, Ltd.) (magnification ranging from ×1,000 to ×10,000). All images were captured on films (Electron-microscopic film FG; Fujifilm).
Dibasic sodium phosphate
Manufactured by Fujifilm
Sourced in Japan
Dibasic sodium phosphate is a chemical compound used as a laboratory reagent. It is a salt of sodium and phosphoric acid, and its primary function is to maintain a specific pH level in various experimental solutions and buffers.
Automatically generated - may contain errors
Lab products found in correlation
0.1 m phosphate buffer, by Fujifilm (1 mentions)
Electron microscopic film fg, by Fujifilm (1 mentions)
Ultracut j microtome, by Reichert Technologies (1 mentions)
Glutaraldehyde, by TAAB (1 mentions)
Jem 1200ex 2, by JEOL (1 mentions)
Osmium tetroxide, by Nisshin EM (1 mentions)
Gelatin, by Fujifilm (1 mentions)
Quercetin, by Fujifilm (1 mentions)
Ethanol, by Nacalai Tesque (1 mentions)
Sodium azide, by Fujifilm (1 mentions)
Sodium dihydrogen phosphate, by Fujifilm (1 mentions)
Sodium chloride (nacl), by Nacalai Tesque (1 mentions)
Potassium dihydrogen phosphate, by Nacalai Tesque (1 mentions)
Sodium bicarbonate, by Nacalai Tesque (1 mentions)
Silica gel 60n, by Kanto Chemical (1 mentions)
Sulphuric acid, by Fujifilm (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (1 mentions)
Dl glyceraldehyde, by Fujifilm (1 mentions)
O phenylenediamine dihydrochloride, by Merck Group (1 mentions)
2 protocols using dibasic sodium phosphate
1
Ultrastructural Analysis of Cells
2
Inhibition of Aldose Reductase by Natural Compounds
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Dibasic sodium phosphate, sodium dihydrogen phosphate, D,L-glyceraldehyde, human recombinant aldose reductase (HR-AR), AG, quercetin, critric acid monohydrate, natrium carbonicum, sodium azide, gelatin and sulphuric acid were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Sodium bicarbonate, sodium chloride, potassium dihydrogen phosphate and ethanol were supplied by Nacalai Inc. (Kyoto, Japan). Tween 20, bovine serum albumin, glucose, O-phenylenediamine dihydrochloride, phosphate buffered saline, fetal bovine serum (FBS), steroyl myristoyl phosphatidylcholine glycine (SMPC Gly), 3-(4, 5-dimethylthazol-2-yl)-2, 5-diphenyl tetrazolium bromidetetrazolium salt (MTT) were purchased from Sigma-Aldrich company, Ltd. (St. Louis, MO, U.S.A.). NADPH was provided by Oriental Yeast Co., Ltd. (Tokyo, Japan). Anti-AGE antibody and goat anti-mouse IgG, HRP conjugate-secondary antibody Millipore (Merck U.S.A) were purchased from Transgenic Inc. (Hyogo, Japan). Silica gel 60 F254 TLC plates (Merck, U.S.A), silica gel 60 N (100–200 μm) and ODS were purchased from Kanto Chemical (Tokyo, Japan). DMSO was purchased from Wako Pure Chemical Industries, Ltd (Japan). Kaempferol-3-O-β-D -glucopyranoside was isolated from liquorice, and was identified by NMR and MS data [11 (link)].
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