A stock of 5 mg ml−1 of ciprofloxacin in sterile, de-ionized water was prepared with ciprofloxacin hydrochloride (MP Biomedicals, Santa Ana, CA, USA) and mixed thoroughly. Tobramycin stock was made in a similar fashion by mixing 5 mg ml−1 of Tobramycin sulfate salt (Sigma-Aldrich, St Louis, MO, USA) in sterile, de-ionized water. Erythromycin was obtained from MP Biomedicals and the stock was prepared by mixing 5 mg m ml−1 Erythromycin into ethanol as directed. Each antibiotic mixture was then filter sterilized through a 0.22 μl PES membrane sterile filter (Millex®GP filter unit) and stored at 2 °C. Each antibiotic was then diluted from the stock solution into sterile TSB at an array of concentrations in a 96-well challenge plate used that day.
Tobramycin sulfate salt
Manufactured by Merck Group
Sourced in United States
Tobramycin sulfate salt is a laboratory reagent used as a standard in various analytical procedures. It is a water-soluble powder that can be used as a reference compound for the identification and quantification of tobramycin in samples.
Automatically generated - may contain errors
Lab products found in correlation
Ciprofloxacin hydrochloride, by MP Biomedicals (2 mentions)
Tobramycin, by Merck Group (2 mentions)
Erythromycin, by MP Biomedicals (2 mentions)
Ciprofloxacin, by MP Biomedicals (2 mentions)
Ampicillin sodium salt, by Thermo Fisher Scientific (1 mentions)
Neomycin trisulfate salt hydrate, by Merck Group (1 mentions)
Kanamycin sulfate, by Thermo Fisher Scientific (1 mentions)
Streptomycin sulfate, by Thermo Fisher Scientific (1 mentions)
Gentamicin sulfate, by Merck Group (1 mentions)
Amikacin disulfate salt, by Merck Group (1 mentions)
L glutathione reduced, by Merck Group (1 mentions)
3 protocols using tobramycin sulfate salt
1
Preparation and Dilution of Antibiotic Stocks
2
Preparation of Antibiotic Stock Solutions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A total of 5 mg mL−1 ciprofloxacin stock was prepared by dissolving ciprofloxacin hydrochloride (MP Biomedicals, Santa Ana, CA, USA) in deionized water. Tobramycin stock was prepared following the same procedure by dissolving Tobramycin sulfate salt (Sigma Aldrich, St. Louis, MO, USA). Erythromycin was obtained from MP Biomedicals, and the stock was prepared by mixing 1.5 mg mL−1 Erythromycin into deionized water. Stocks were filtrated using a 0.22 μm pore PES membrane sterile filter (Millex®GP filter unit) and stored at 2 °C.
3
Antibiotics and Reagents for Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sanguinarine chloride (Aladdin, S1010540，Shanghai, China). l ‐Glutathione reduced (Sigma, G4251, Darmstadt, Germany). Kanamycin sulfate (Thermo Fisher Scientific, J17924.06, Carlsbad, California, USA). Tobramycin sulfate salt (Sigma, T1783, Darmstadt, Germany). Gentamicin sulfate (Sigma, G1914, Darmstadt, Germany). Streptomycin sulfate (Fisher Scientific, BP910‐50, Carlsbad, California, USA). Neomycin trisulfate salt hydrate (Sigma, N1876). Amikacin disulfate salt (Sigma, A1774, Darmstadt, Germany). Ampicillin sodium salt (Fisher Scientific, BP1760, Carlsbad, California, USA). Norfloxacin (MP Biomedicals, 155949, Solon, Ohio, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!