Protein g agarose 16 266

DMEM (10100147) and fetal bovine serum (C11995500BT) were from Gibco. MG132 (HY-13259) and protease-inhibitor cocktail (HY-K0010) were from MCE. Lipofectamine™ 3000 Transfection Reagent (L3000015) was from Invitrogen. Cycloheximide (CHX) and N-ethylmaleimide (NEM) were from Sigma-Aldrich. The following antibodies were from Abcam: anti-E4B (ab126759), anti-ubiquitin (ab134953), anti-HA (ab182009). Anti-GAPDH antibody (60004-1-Ig) and anti-PYCR2 antibody (17146-1-AP) were from Proteintech. Anti-TRA2A antibody (GTX87998) was from GeneTex. Anti-MYC antibody (9B11) was from Cell Signaling Technology. Anti-FLAG (M2) antibody (F1804) and anti-FLAG(M2) affinity gel (A2220) were from Sigma-Aldrich. Goat anti-rabbit IgG-Alexa Fluor 790 antibody (111-655-144) and goat anti-mouse IgG- Alexa Fluor 790 antibody (115-005-072) were from Jackson ImmunoResearch. Protein G Agarose (16-266) was from Merckmilipore. Phenylmethanesulfonyl fluoride (PMSF, ST506) and RIPA Lysis Buffer (P0013C) were from Beyotime. Polyetherimide (AC04L091) was from Life-iLab.

Apilimod (No. S6414) was bought from Selleck. Nigericin, MSU, and poly (A/T) were purchased from Sigma-Aldrich. Ultrapure LPS, MitoSOX, MitoTracker, DAPI, Lysotracker Green, MitoTracker Deep Red, MitoTracker Green were acquired from Invitrogen. MnTBAP was bought from Santa Cruz Biotechnology. The C3 toxin was a gift from Dr. Tengchuan Jin (University of Science and Technology of China, Hefei, China). CY-09 was synthesized from Dr. Xianming Deng (Xiamen University, Xiamen, China). MCC950 was bought from Selleck. Fluo-4, AM(HY-101896), ML-SI3 were purchased from MedChemExpress. BAPTA-AM(A1076) was bought from Sigma-Aldrich. Protein G agarose (16–266) was bought from Millipore. Anti-Flag (F2555) antibodies were from Sigma-Aldrich. The anti-mouse NLRP3 (AG-20B-0014) and the anti-mouse caspase-1 (AG-20B-0042) antibodies was from AdipoGen. The anti-mouse IL-1β antibody (AF-401-NA) was obtained from R&D Systems. The anti-β-actin antibody (66009-1-Ig) was from Proteintech Group. The anti-mouse ASC antibody (67824S) was from Cell Signaling Technology. The anti-mouse NEK7 antibody (ab133514) was from Abcam.

ATP, Nigericin, MSU, and poly (A/T) were purchased from Sigma-Aldrich. Ultrapure LPS, Lipofextamine 2000, Pam3CSK4, MQAE, MitoSOX, MitoTracker, DAPI were acquired from Invitrogen. The Salmonella strain was a gift from Dr. Cai Zhang (Shandong University, Shandong, China). The C3 toxin was a gift from Dr. Tengchuan Jin (University of Science and Technology of China, Hefei, China). CY-09 was synthesized from Dr. Xianming Deng (Xiamen University, Xiamen, China). Protein G agarose (16-266) and anti-Flag (A2220) beads were bought from Millipore and Sigma-Aldrich, respectively. Anti-Flag (F2555) and anti-VSV (V4888) antibodies were from Sigma-Aldrich. The anti-mouse NLRP3 (AG-20B-0014) and the anti-mouse caspase-1 (AG-20B-0042) antibodies was from AdipoGen. The anti-mouse IL-1β antibody (AF-401-NA) was obtained from R&D Systems. The anti-β-actin antibody (66009-1-Ig) was from Proteintech Group. The anti-mouse ASC antibody (67824S) was from Cell Signaling Technology. The anti-mouse NEK7 antibody (ab133514) was from Abcam.