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Protease inhibitor cocktails set 3

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Protease inhibitor cocktails set III is a collection of reagents designed to inhibit the activity of proteases, enzymes that break down proteins. This set is intended for use in research applications that require the preservation of protein integrity during sample preparation and analysis.

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2 protocols using protease inhibitor cocktails set 3

1

Protein Extraction from Cell Lysates

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Cells were harvested and centrifugated at 500×g for 10 min. The pellets were resuspended in a cold lysis buffer (P0013B, Beyotime, China) supplemented with the protease inhibitor cocktails set III (535140, Merck, United States), and maintained on ice for 30 min. The supernatants were collected and the protein concentrations were determined by a BCA method (71285-3, Merck, United States).
Hu C., Chen C., Xia Y., Chen J., Yang W., Wang L., Chen D.D., Wu Y.Z., Fan Q., Jia X.X., Xiao K., Shi Q., Chen Z.B, & Dong X.P. (2022). Different Aberrant Changes of mGluR5 and Its Downstream Signaling Pathways in the Scrapie-Infected Cell Line and the Brains of Scrapie-Infected Experimental Rodents. Frontiers in Cell and Developmental Biology, 10, 844378.
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2

Cell Lysis and Protein Extraction

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Cells were harvested and centrifugated with low speed centrifugation at 500×g for 10 min. The pellets were resuspended in a cold lysis buffer (P0013B, Beyotime, China) supplemented with the protease inhibitor cocktails set III (535140, Merck, USA), and maintained on ice for 30 min. The supernatants were harvested and aliquoted for further study after determination of the protein concentration by a BCA method (71285-3, Merck, USA).
Jia X.X., Chen C., Hu C., Chao Z.Y., Zhang W.W., Wu Y.Z., Fan Q., A R.H., Liu X., Xiao K., Shi Q, & Dong X.P. (2023). Abnormal Changes of IL3/IL3R and Its Downstream Signaling Pathways in the Prion-Infected Cell Line and in the Brains of Scrapie-Infected Rodents. Molecular neurobiology.
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