FAAH inhibitor URB597 (Cyclohexylcarbamic acid 3′-[aminocarbonyl]-[1,1′-biphenyl]-3-yl ester) and selective CB1 antagonist AM251 [Tocris Inc., Bristol, UK] were dissolved in 1% DMSO plus physiological saline and in 5% Cremophor in physiological saline, respectively. Alcohol solutions (7.5%, 15% and 30% v/v) were prepared from 190 proof absolute ethyl alcohol (Pharmco-AAPER, Brookfield, CT, USA) and dissolved in tap water. Sucrose and saccharin [Sigma-Aldrich Inc., St. Louis, MO, USA) were diluted in tap water. Optima liquid chromatography-mass spectrometry (LC/MS) grade acetonitrile [Fisher Scientific, Pittsburgh, PA, USA] was used for metabolite extractions and liquid chromatography. Deuterated anandamide standard was purchased from Cayman Chemical [Ann Arbor, Michigan, USA].
Sucrose and saccharin
Manufactured by Merck Group
Sourced in United States
Sucrose and saccharin are two commonly used lab equipment products. Sucrose is a disaccharide sugar composed of glucose and fructose, while saccharin is an artificial sweetener. Both are used in various laboratory applications, but a detailed description of their core functions would require more specific information about the intended use, which is not provided. Therefore, a concise, unbiased, and factual description cannot be presented without the risk of extrapolation or interpretation.
Automatically generated - may contain errors
4 protocols using sucrose and saccharin
1
Endocannabinoid Modulation Protocols
2
Rodent Habituation and Reward Training
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For the first 7–10 days rats were habituated to the experimenter. During this period, rats were handled frequently and introduced to new types of foods and flavored water which were later used as rewards in the experimental arena. The procedure was aimed to decrease stress and prevent neophobia towards rewards given during behavioral training. Rewards consisted of palatable 45 mg food tablets in six flavors: basic company flavor, banana, bacon, chocolate, peanut butter, and fruit punch (diet AIN-76A: RodTab45MG; RodTabBan45MG; RodTabBcn45MG; RodTabChoc45MG; RodTabPbtr45MG; RodTabFrtP45MG5TUL, TestDiet, Richmond, IN, USA). Rats received also 4 types of fluids: mineral water, 4% sucrose in mineral water, 0.1% saccharine in mineral water, and 1:1 mixture of 4% sucrose, and 0.1% saccharine solutions (sucrose and saccharin, Sigma-Aldrich, St. Louis, MO, USA).
In order to motivate animals to work for food, rats were food-restricted up to 85% of their ad libitum body weight. Rats were subjected also to mild water restriction before behavioral sessions (4–12h). Experiments were carried out 5 days a week. During the weekend, animals had free access to standard rodent food and unsweetened mineral water.
In order to motivate animals to work for food, rats were food-restricted up to 85% of their ad libitum body weight. Rats were subjected also to mild water restriction before behavioral sessions (4–12h). Experiments were carried out 5 days a week. During the weekend, animals had free access to standard rodent food and unsweetened mineral water.
3
Preparation of Drug and Alcohol Solutions
4
Reward-Based Behavioral Training in Rats
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For the first 7-10 days rats were habituated to the experimenter. During this period, rats were handled frequently and introduced to new types of foods and flavored water which were later used as rewards in the experimental arena. The procedure was aimed to decrease stress and prevent neophobia towards rewards given during behavioral training. Rewards consisted of palatable 45 mg food tablets in six flavors: basic company flavor, banana, bacon, chocolate, peanut butter and fruit punch (diet AIN-76A: RodTab45MG; RodTabBan45MG; RodTabBcn45MG; RodTabChoc45MG; RodTabPbtr45MG; RodTabFrtP45MG5TUL, TestDiet, Richmond, IN, USA). Rats received also 4 types of fluids: mineral water, 4% sucrose in mineral water, 0.1% saccharine in mineral water, and 1:1 mixture of 4% sucrose and 0.1 % saccharine solutions (sucrose and saccharin, Sigma-Aldrich, St. Louis, MO, USA).
In order to motivate animals to work for food, rats were food-restricted up to 85% of their ad libitum body weight. Rats were subjected also to mild water restriction before behavioral sessions (4-12h). Experiments were carried out 5 days a week. During the weekend animals had free access to standard rodent food and unsweetened mineral water.
In order to motivate animals to work for food, rats were food-restricted up to 85% of their ad libitum body weight. Rats were subjected also to mild water restriction before behavioral sessions (4-12h). Experiments were carried out 5 days a week. During the weekend animals had free access to standard rodent food and unsweetened mineral water.
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