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Vybrant apoptosis assay kit 5

Manufactured by Thermo Fisher Scientific
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The Vybrant Apoptosis Assay Kit #5 is a fluorescence-based kit designed to detect and quantify apoptosis in cells. It provides a reliable and sensitive method for measuring cellular viability and apoptosis.

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Lab products found in correlation

S propranolol hydrochloride, by Merck Group (1 mentions) Facsaria, by BD (1 mentions)

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Apoptosis Assay Kit

2 protocols using vybrant apoptosis assay kit 5

1

Propranolol Induces Cell Death

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The 786-O cells were cultured in the presence of 0–200 μM (S)-(−)-propranolol hydrochloride (Sigma-Aldrich) for 24–48 hours at 37°C. The Vybrant Apoptosis Assay Kit #5 (Hoechst 33342/propidium iodide, ThermoFisher Scientific) was then used to determine the percentage of necrotic and apoptotic cells in culture according to the manufacturer’s specification.
Shepard M.J., Bugarini A., Edwards N.A., Lu J., Zhang Q., Wu T., Zhuang Z, & Chittiboina P. (2018). Repurposing propranolol as an antitumor agent in von Hippel-Lindau disease. Journal of neurosurgery.
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2

Assessing Apoptosis and Necrosis

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A2780-CP20 cells (1 × 106/dish) were plated in a 60 mm-dish and incubated with CDDP, CQ, or CQ combined with CDDP for 48 h. After incubation, culture media was collected in 15-ml falcon tubes and cells were washed with 1X cold PBS. Cells were trypsinized, were combined with culture media collected before trypsin treatment, and were washed twice with 1X cold PBS. Cells were incubated with Hoechst33342 and propidium iodide (Vybrant Apoptosis assay kit#5, ThermoFisher) for 10 min. Apoptosis and necrosis were measured with FACS Aria (BD Bioscience).
Hwang J.R., Kim W.Y., Cho Y.J., Ryu J.Y., Choi J.J., Jeong S.Y., Kim M.S., Kim J.H., Paik E.S., Lee Y.Y., Han H.D, & Lee J.W. (2020). Chloroquine reverses chemoresistance via upregulation of p21WAF1/CIP1 and autophagy inhibition in ovarian cancer. Cell Death & Disease, 11(12), 1034.
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