Rabbit monoclonal anti p smad2 antibody

Manufactured by Cell Signaling Technology

Sourced in United States

The Rabbit monoclonal anti-p-Smad2 antibody is a laboratory tool designed to detect the phosphorylated form of the Smad2 protein. Smad2 is a key signaling molecule involved in the transforming growth factor-beta (TGF-β) signaling pathway. This antibody can be used to study the activation and regulation of the TGF-β signaling cascade in various experimental systems.

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2 protocols using rabbit monoclonal anti p smad2 antibody

Immunohistochemical Characterization of Inflammatory Markers

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As primary antibodies, we used a rabbit monoclonal anti-HMGB1 antibody (ab79823; Abcam, Cambridge, UK), rabbit monoclonal anti-IL-18 antibody (ab223293; Abcam), rabbit monoclonal anti-IFN-γ antibody (ab133566; Abcam), mouse monoclonal anti-α-SMA antibody (A-2547; Sigma-Aldrich; St. Louis, MO), mouse monoclonal anti- TGF-β1 antibody (sc-130348; Santa Cruz Biotechnology, Santa Cruz, CA), mouse monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (G8795; Sigma-Aldrich), rabbit monoclonal anti-p-Smad2 antibody (#3108; Cell Signaling Technology, Danvers, MA), mouse monoclonal anti-Smad2 antibody (#3103; Cell Signaling Technology), rabbit polyclonal anti-Col-I antibody (ab6308; Abcam), rabbit polyclonal anti-Col-III antibody (ab7778; Abcam), rabbit polyclonal anti-CD3 antibody (IR503; Dako, Santa Clara, CA), rabbit polyclonal anti-CD68 antibody (ab125212; Abcam), rabbit polyclonal anti-CD206 antibody (ab64693; Abcam) and rabbit monoclonal anti CD163 antibody (ab182422; Abcam).

Kanai R., Nakashima A., Doi S., Kimura T., Yoshida K., Maeda S., Ishiuchi N., Yamada Y., Ike T., Doi T., Kato Y, & Masaki T. (2021). Interferon-γ enhances the therapeutic effect of mesenchymal stem cells on experimental renal fibrosis. Scientific Reports, 11, 850.

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Western Blot Analysis of α-SMA, TGF-β1, and Smad2

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Sample collection and western blotting were performed according to previously described methods [26 (link)]. Mouse monoclonal anti-α-SMA antibody (Sigma-Aldrich), mouse monoclonal anti-TGF-β1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse monoclonal anti-GAPDH antibody (Sigma-Aldrich), rabbit monoclonal anti-p-Smad2 antibody (Cell Signaling Technology, Danvers, MA, USA), mouse monoclonal anti-Smad2 antibody (Cell Signaling Technology), and mouse monoclonal anti-α-Tubulin antibody (Sigma-Aldrich) were used as primary antibodies. Horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (Dako, Glostrup, Denmark) or goat anti-mouse immunoglobulin G (Dako) was used as secondary antibodies. SuperSignal West Dura or the Pico system (Thermo Fisher Scientific, Rockford, IL, USA) were used to detect signals. The intensity of each band was measured by ImageJ software (version 1.47v; National Institutes of Health) and normalized to the level of either GAPDH or α-tubulin.

Ishiuchi N., Nakashima A., Doi S., Yoshida K., Maeda S., Kanai R., Yamada Y., Ike T., Doi T., Kato Y, & Masaki T. (2020). Hypoxia-preconditioned mesenchymal stem cells prevent renal fibrosis and inflammation in ischemia-reperfusion rats. Stem Cell Research & Therapy, 11, 130.

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