Thermo nicolet avatar 360

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Thermo Nicolet Avatar 360 is a Fourier Transform Infrared (FTIR) spectrometer. It is designed to analyze the chemical composition and structural properties of a wide range of materials through infrared spectroscopy.

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Lab products found in correlation

D8 advance diffractometer, by Bruker (1 mentions)

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Avatar 360 Thermo Nicolet Spectrometer Nicolet Avatar 360 Avatar 360 Nicolet-360

4 protocols using thermo nicolet avatar 360

Structural Analysis of Budesonide Nanoformulations

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The diffraction patterns of budesonide, budesonide-loaded nanoparticles, empty nanoparticles, and Eudragit L100 were collected from 5 to 80°2θ on a Bruker D8-Advance Diffractometer (Karlsruhe, Germany). CuKα radiation was used, and registration was performed by the LynxEye detector. The unit cell parameters were refined using the Topas 4.2 program, part of the Bruker software (Bruker AXS, Karsruhe, Germany).
The FTIR spectra of budesonide, budesonide-loaded nanoparticles, empty nanoparticles, and Eudragit L100 in KBr were recorded on a Thermo Nicolet Avatar 360 FTIR spectrometer (Thermo Fisher Scientific, Waltham, MA, USA), within the range 4000–400 cm⁻¹ with a resolution of 2 cm⁻¹.

Slavkova M., Lazov C., Spassova I., Kovacheva D., Tibi I.P., Stefanova D., Tzankova V., Petrov P.D, & Yoncheva K. (2024). Formulation of Budesonide-Loaded Polymeric Nanoparticles into Hydrogels for Local Therapy of Atopic Dermatitis. Gels, 10(1), 79.

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Characterization of Ca-Alg Particles

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The Ca-Alg particles were imaged with upright microscope #1 Olympus BX51 (Olympus, Tokyo, Japan) and inverted fluorescence microscope #2 Olympus IX71 (Olympus, Tokyo, Japan). Particle diameters (average diameter) and aspect ratios were calculated using microscope images with “Analyze Particles” plugin of image processing software “ImageJ 1.51J8”.^30,31 In this software plugin, one can automatically determine the particle size and aspect ratio, if the pixel size is set in ImageJ. The degree of irregularity is displayed by the aspect ratio where e.g. long particles show a higher aspect ratio than spherical particles. The average diameter and standard error was calculated via Microsoft Excel 2016. Zeta-Potential measurements were done with a ZetaPALS – Zeta Potential Analyzer (Brookhaven Instruments Cooperation, Holtsville, NY, USA) and specified with ζ = −19.78 ± 1.4 mV, which is in agreement with ref. 32 . FT-IR Spectra of Na-Alg and Ca-Alg were made with Thermo Nicolet Avatar 360 (Thermo Fisher Scientific, Waltham, MA, USA) FTIR Spectrometer ((ESI), Fig. S1†). FTIR spectrum of Ca-Alg in ESI, Fig. S1B† shows significant changes compared to the pristine Na-Alg FTIR spectrum displayed in ESI, Fig. S1A.† This proves a successful cross-linking of alginate (ALG) with Ca²⁺ ions (ESI, Fig. S2†).

Rutkowski S., Si T., Gai M., Frueh J, & He Q. (2018). Hydrodynamic electrospray ionization jetting of calcium alginate particles: effect of spray-mode, spraying distance and concentration. RSC Advances, 8(43), 24243-24249.

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Fourier Transform Infrared Characterization

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To perform the Fourier transform infrared characterization, the spectra were collected in a Thermo Nicolet Avatar 360 IR spectrophotometer (Thermo Electron Inc., Waltham, MA, USA) with a resolution of 4 cm⁻¹, equipped with a DTGS detector and OMNIC software 7.2 (Thermo Nicolet, Waltham, MA, USA). Baseline adjustment was performed using Thermo Nicolet OMNIC (Thermo Nicolet, Waltham, MA, USA) software to flatten the baseline in each spectrum. For this, a self-supporting disc had been previously made, pressed from the mixture of EgPs-KBr (1% p/p), which was introduced in the spectrophotometer for its characterization.

Casas-Arrojo V., Arrojo Agudo M.D., Cárdenas García C., Carrillo P., Pérez Manríquez C., Martínez-Manzanares E, & Abdala Díaz R.T. (2022). Antioxidant, Immunomodulatory and Potential Anticancer Capacity of Polysaccharides (Glucans) from Euglena gracilis G.A. Klebs. Pharmaceuticals, 15(11), 1379.

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Characterization of Polysaccharide Structure by FT-IR

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FT-IR analysis was used to characterize the PcSPs structure and to identify the functional groups in the PcSPs structure [32 (link)]. The FT-IR spectra in the 400–4000 cm⁻¹ region were obtained using self-supporting pressed disks of 16 mm in diameter of a mixture of polysaccharides and KBr (1% w/w) with a hydrostatic press at a force of 15.0 tcm⁻² for 2 min. Thermo Nicolet Avatar 360 IR spectrophotometer (Thermo Electron Inc., Franklin, MA, USA) having a resolution of 4 cm⁻¹ with a DTGS detector operated with OMNIC 7.2 software (bandwidth 50 cm⁻¹, enhancement factor 2.6). Baseline adjustment was performed using the Thermo Nicolet OMNIC software 9 (Termo Fischer, San Jose, CA, USA). The OMNIC correlation algorithm was used to compare sample spectra with those of the spectral library (Thermo Fischer Scientific).

Casas-Arrojo V., Decara J., de los Ángeles Arrojo-Agudo M., Pérez-Manríquez C, & Abdala-Díaz R.T. (2021). Immunomodulatory, Antioxidant Activity and Cytotoxic Effect of Sulfated Polysaccharides from Porphyridium cruentum. (S.F.Gray) Nägeli. Biomolecules, 11(4), 488.

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