In this study, the human cervical cancer cell lines, SiHa cells and HeLa cells, and the NK-92 cells were from a research laboratory of Foshan Maternity and Child Health Care Hospital, Guangdong Province, China. The SiHa and HeLa cells were cultured in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal bovine serum (Invitrogen, Carlsbad, CA, USA) and 1% streptomycin (Sigma-Aldrich, St. Louis, MO, USA). The NK-92 cells were cultured in alpha-minimum essential medium (ɑ-MEM) containing 100 U/mL interleukin (IL)-2. Dimethyl sulfoxide (DMSO), LY294002, and metformin hydrochloride were purchased from Sigma-Aldrich. Anti-MICA, anti-MICB, anti-ULBP-2/5/6 Phycoerythrin, anti-ULBP-1 Alexa Fluor 488, anti-ULBP-3 Allophycocyanin, anti-DDR-1, anti-HSF-1, anti-PI3K (110ɑ), anti-phospho-PI3Kp55 (Tyr199), anti-Akt, anti-phospho-Akt (ser473), anti- proliferating cell nuclear antigen (PCNA), anti-p53 primary antibodies, and their corresponding secondary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).
Anti p53 primary antibodies
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-p53 primary antibodies are used to detect the p53 protein, which is a key regulator of cellular processes such as cell cycle, apoptosis, and DNA repair. These antibodies can be used in various immunoassays and immunohistochemical applications to study the expression and localization of p53 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
Streptomycin, by Merck Group (3 mentions)
Ly294002, by Merck Group (3 mentions)
Anti ulbp 2 5 6 phycoerythrin, by Cell Signaling Technology (3 mentions)
Anti ulbp 1 alexa fluor 488, by Cell Signaling Technology (3 mentions)
Anti ulbp 3 allophycocyanin, by Cell Signaling Technology (3 mentions)
Metformin hydrochloride, by Merck Group (3 mentions)
Anti hsf1, by Cell Signaling Technology (3 mentions)
Anti ddr1, by Cell Signaling Technology (3 mentions)
Anti pi3k 110, by Cell Signaling Technology (2 mentions)
Anti micb, by Cell Signaling Technology (1 mentions)
3 protocols using anti p53 primary antibodies
1
Modulation of NK-92 Cell Cytotoxicity Against Cervical Cancer
2
Modulation of NK-92 Cell Cytotoxicity against Cervical Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In this study, the human cervical cancer cell lines, SiHa cells and HeLa cells, and the NK-92 cells were from a research laboratory of Foshan Maternity and Child Health Care Hospital, Guangdong Province, China. The SiHa and HeLa cells were cultured in Dulbecco's modi ed eagle medium (DMEM) containing 10% fetal bovine serum (Invitrogen, Carlsbad, CA, USA) and 1% streptomycin (Sigma-Aldrich, St. Louis, MO, USA). The NK-92 cells were cultured in alpha-minimum essential medium ( -MEM) containing 100 U/mL interleukin (IL)-2. Dimethyl sulfoxide (DMSO), LY294002, and metformin hydrochloride were purchased from Sigma-Aldrich. Anti-MICA, anti-MICB, anti-ULBP-2/5/6 Phycoerythrin, anti-ULBP-1 Alexa Fluor 488, anti-ULBP-3 Allophycocyanin, anti-DDR-1, anti-HSF-1, anti-PI3K (110 ), anti-phospho-PI3Kp55 (Tyr199), anti-Akt, anti-phospho-Akt (ser473), anti-proliferating cell nuclear antigen (PCNA), anti-p53 primary antibodies, and their corresponding secondary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).
3
Modulation of NK-92 Cell Cytotoxicity against Cervical Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In this study, the human cervical cancer cell lines, SiHa cells and HeLa cells, and the NK-92 cells were from a research laboratory of Foshan Maternity and Child Health Care Hospital, Guangdong Province, China. The SiHa and HeLa cells were cultured in Dulbecco's modi ed eagle medium (DMEM) containing 10% fetal bovine serum (Invitrogen, Carlsbad, CA, USA) and 1% streptomycin (Sigma-Aldrich, St. Louis, MO, USA). The NK-92 cells were cultured in alpha-minimum essential medium ( -MEM) containing 100 U/mL interleukin (IL)-2. Dimethyl sulfoxide (DMSO), LY294002, and metformin hydrochloride were purchased from Sigma-Aldrich. Anti-MICA, anti-MICB, anti-ULBP-2/5/6 Phycoerythrin, anti-ULBP-1 Alexa Fluor 488, anti-ULBP-3 Allophycocyanin, anti-DDR-1, anti-HSF-1, anti-PI3K (110 ), anti-phospho-PI3Kp55 (Tyr199), anti-Akt, anti-phospho-Akt (ser473), anti-proliferating cell nuclear antigen (PCNA), anti-p53 primary antibodies, and their corresponding secondary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).
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