F4 80 bv421 clone bm8

The SVCs were preincubated with mouse BD FcR block (clone 2.4G2, BD Biosciences) for 5 min at 4°C and then incubated with fluorescent-labeled monoclonal anti-mouse antibodies (F4/80 BV421 clone BM8, CD206 FITC clone C068C2, CD301 PE clone LOM-14 from BioLegend; CD86 APC clone B7-2 from BD Biosciences), or respective immunoglobulin controls (immunoglobulins and antibodies dilution 1:100) in the FACS buffer for 30 min at 4°C in the dark. The cells were washed by adding PBS and centrifuged at 1,300 rpm for 10 min at 4°C); the labeled cells were fixed using BD CellFIX (BD Biosciences) and analyzed by flow cytometry using a FACS Verse flow cytometer (BD Biosciences) and FlowJo software.

CD3-FITC clone 145-2C11, CD4-APC-Cy7 clone RM4-5, CD8-FITC clone 53-6.7, CD45-AF700 clone 104, CD11b-PE-Cy7 clone M1/70, CD11c-BV450 clone N418, CD19-PerCP-Cy5.5 clone 6D5, CD49b-APC clone DX5, F4/80-BV421 clone BM8, IL-17A-PE-Cy7 clone TC11-18H10.1, Ly6G-APC clone 1A8, and TCRγδ-BV421 clone GL3, and were purchased from Biolegend (San Diego, CA). Ly6C-PerCP-Cy5.5 clone AL-21, RORγt-BV650 clone Q31-378, and Siglec F-PE clone E50-2440 were purchased from BD Biosciences (San Jose, CA). Foxp3-PE clone 150D/E4 was purchased from ThermoFisher Scientific (Waltham, MA).