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3 protocols using menthyl acetate

1

Enantiomeric Analysis of Essential Oils

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For chemical analyses, EOs were diluted in cyclohexane MS SupraSolv® for gas chromatography (Sigma-Aldrich, Steinheim, Germany). For enantiomers identification, the analytical standards of (−)-menthol, (+)-menthol, (−)-menthone, (+)-menthone, (−)-α-terpineol, (+)-α-terpineol, (−)-limonene, (+)-limonene, (−)-terpinen-4-ol, (+)-terpinen-4-ol, (−)-trans-caryophyllene, (−)-carvone, (+)-carvone, (−)-linalool, (±)-linalool, (−)-menthyl acetate, (+)-menthyl acetate, (−)-dihydrocarvone, (+)-dihydrocarvone, (−)-borneol, and undecane-2-one (all Sigma-Aldrich, Steinheim, Germany) were used.
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2

Headspace GC-MS Analysis of Menthol Compounds

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Headspace GC-MS analysis was performed on an Agilent 7890B gas chromatograph equipped with an Agilent 5977 single-quadrupole mass spectrometer (Agilent Technologies, Amstelveen, the Netherlands) and a multipurpose sampler (MPS-2, Gerstel, Mühlheimander Ruhr, Germany). -Menthol, menthone and menthyl acetate were of analytical grade and purchased from Sigma-Aldrich (Zwijndrecht, the Netherlands).
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3

Headspace GC-MS Analysis of Terpenes

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The reference compounds (-)-menthol, menthone, menthyl acetate, and p-xylene-d10 were of analytical grade and purchased from Sigma-Aldrich (Zwijndrecht, the Netherlands). Methanol came from Biosolve BV (Valkenswaard, the Netherlands).
Headspace GC-MS analysis was performed on an Agilent 7890B gas chromatograph equipped with a 5977 single quadrupole mass spectrometer (Agilent Technologies, Amstelveen, the Netherlands) and a multi-purpose sampler (MPS-2, Gerstel, Mühlheimander Ruhr, Germany).
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