Transwell chambers with 0.4 μm pores

Manufactured by Corning

Sourced in United States

Transwell chambers with 0.4-μm pores are a laboratory equipment product designed for cell culture applications. These chambers feature a porous membrane with a pore size of 0.4 micrometers, which allows for the study of cellular interactions and transport processes across a barrier.

Automatically generated - may contain errors

Lab products found in correlation

Gw4869, by Merck Group (1 mentions) Gw4869, by MedChemExpress (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)

Close spelling variants of this product

0.4 μm pore Transwell chamber 0.4 μm pore transwell (0.4-μm pore, Transwell 0.4 μm Pore transwells Transwell chambers

2 protocols using transwell chambers with 0.4 μm pores

Macrophage-RCC Exosome Secretion Assay

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Macrophages were seeded in Transwell chambers with 0.4-μm pores (Corning Life Science, NY, USA), and RCC cells were seeded in the bottom of a 6-well plate. After 2 days of coculture, RCC cells and conditioned medium (CM) were harvested for further experiments. Exosome secretion inhibitor GW4869 (MedChemExpress, Monmouth Junction, NJ, USA) was employed for CM treatment at 5 μM concentration. DMSO (Sigma) was used as the control for GW4869.

Liu Q., Zhao E., Geng B., Gao S., Yu H., He X., Li X., Dong G, & You B. (2022). Tumor-associated macrophage-derived exosomes transmitting miR-193a-5p promote the progression of renal cell carcinoma via TIMP2-dependent vasculogenic mimicry. Cell Death & Disease, 13(4), 382.

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Co-culture of ADMSCs and M1 BMDMs

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Transwell chambers with 0.4 μm pores (Corning, California, USA) were used in the co-culture experiments of ADMSCs and BMDMs. Before co-cultured with ADMSCs, BMDMs were polarized to M1 phenotype with the stimulation of 100 ng/mL LPS (MCE, Monmouth Junction, NJ, USA) and 20 ng/mL hIFN-γ (MCE) for 48 hours. Then ADMSCs and BMDMs were planted onto transwell inserts at the ratio of 1:1 and co-cultured for 24 hours before assessment.

Yuan Y., Ni S., Zhuge A., Li L, & Li B. (2022). Adipose-Derived Mesenchymal Stem Cells Reprogram M1 Macrophage Metabolism via PHD2/HIF-1α Pathway in Colitis Mice. Frontiers in Immunology, 13, 859806.

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